Production of α-1,3-galactosyltransferase knockout pigs by nuclear transfer coning
Production of α-1,3-galactosyltransferase knockout pigs by nuclear transfer coning
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DOI：
10.1126/science.1068228
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发表时间：
2002-02-08
期刊：
影响因子：
56.9
通讯作者：
Prather, RS
中科院分区：
文献类型：
--
作者：
Lai, LX;Kolber-Simonds, D;Prather, RS
猪细胞表面存在α-1，3-半乳糖残基是异种移植成功的主要障碍。在这里，我们报告了四头生猪的生产，其中α-1，3-半乳糖基转移酶基因的一个等位基因已经被敲除。这些猪是通过核移植技术生产的，克隆的胎儿成纤维细胞系作为核供体，用去核的猪卵母细胞重建胚胎。
The presence of galactose alpha-1,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the alpha-1,3-galactosyltransferase locus has been knocked out. These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes.