Genetic and evolutionary characterization of norovirus from sewage and surface waters in Cordoba City, Argentina
Genetic and evolutionary characterization of norovirus from sewage and surface waters in Cordoba City, Argentina
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DOI：
10.1016/j.meegid.2011.06.005
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发表时间：
2011-10-01
影响因子：
3.2
通讯作者：
Mbayed, Viviana A.
中科院分区：
文献类型：
--
作者：
Blanco Fernandez, Maria D.;Torres, Carolina;Mbayed, Viviana A.
诺如病毒（NoV）是在全世界所有年龄段的人类中引起胃肠炎的最常见的病毒因子之一。它们随粪便排出，并作为未经处理或经处理的污水进入环境沃茨。在这项工作中，污水和水样本收集的苏基亚河在科尔多瓦市，阿根廷，进行了评估的存在NoV.系统发育分析表明，检测到的主要基因型是GII. 4，属于广泛分布的2006年b的变体，其次是菌株相关的推定重组GII.g病毒。检测到的NoV与来自其他国家的最近病毒的遗传相关性比与以前的本地序列更高，表明病毒株的快速和广泛传播，防止了地理结构的遗传。贝叶斯结合分析表明，在这项工作中分离的变异体具有最近的共同祖先，位于2007-2008年，估计替代率为3.7-5.8 x 10（-3）s/s/y。环境样本显示两种病毒类型的混合物，指出了共同循环以及混合感染和重组的风险。这是关于在阿根廷的污水和河水中检测和表征NoV的第一份报告。(C)2011爱思唯尔有限公司版权所有。
Noroviruses (NoVs) are among the most common viral agents that cause gastroenteritis in humans of all ages worldwide. They are excreted in the feces and introduced into environmental waters as raw or treated sewage. In this work, sewage and water samples collected from the Suquia River in the city of Cordoba, Argentina, were evaluated for the presence of NoV. Phylogenetic analysis demonstrated that the main genotype detected was GII.4, belonging to the widely-distributed 2006b variant, followed by strains related to the putative recombinant GII.g virus. Detected NoVs were more phylogenetically related with recent viruses from other countries than with previous local sequences, suggesting a rapid and wide spread of viral strains that prevents a geographically structured phylogeny. A Bayesian coalescent analysis demonstrated that variants isolated in this work have a most recent common ancestor placed in 2007-2008 with estimated substitution rates of 3.7-5.8 x 10(-3) s/s/y. Environmental samples showed a mixture of both viral types, pointing up to the co-circulation and the risk of mixed infections and recombination. This is the first report on the detection and characterization of NoV in sewage and river water in Argentina. (C) 2011 Elsevier B.V. All rights reserved.