CAPACITY OF AQUATIC BACTERIA TO ACT AS RECIPIENTS OF PLASMID DNA
CAPACITY OF AQUATIC BACTERIA TO ACT AS RECIPIENTS OF PLASMID DNA
复制标题
DOI：
10.1128/aem.54.1.115-117.1988
复制
发表时间：
1988-01-01
影响因子：
4.4
通讯作者：
BOURQUIN, AW
中科院分区：
文献类型：
--
作者：
GENTHNER, FJ;CHATTERJEE, P;BOURQUIN, AW
共筛选了68株革兰氏阴性淡水细菌分离株接受和表达铜绿假单胞菌供体质粒的能力。平板交配技术确定了26个菌株作为受体活性的自我传播的广宿主范围质粒R68; 10个受体活性的R68动员的广宿主范围质粒克隆载体R1162。通过使用三种接合转移程序（肉汤、平板和过滤器交配）比较转移频率。对于每个接受测试的受体，固体环境上级液体环境进行转移。肉汤交配技术未能证明63%的毒力活性分离株中存在R68转移。一般来说，滤波器配对产生最高的传输频率。然而，更快速的平板交配程序对于测试天然分离物参与接合质粒转移的能力同样敏感。
A total of 68 gram-negative freshwater bacterial isolates were screened for their ability to receive and express plasmids from Pseudomonas aeruginosa donors. The plate mating technique identified 26 of the isolates as recipient active for the self-transmissible wide-host-range plasmid R68; 10 were recipient active by R68 mobilization for the wide-host-range plasmid cloning vector R1162. Frequencies of transfer were compared by using three conjugal transfer procedures: broth, plate, and filter mating. For every recipient tested, a solid environment was superior to a liquid environment for transfer. The broth mating technique failed to demonstrate R68 transfer in 63% of the recipient-active isolates. Filter mating, in general, yielded the highest transfer frequencies. The more-rapid plate mating procedure, however, was just as sensitive for testing the capacity of natural isolates to participate in conjugal plasmid transfer.