Core histone N-termini play an essential role in mitotic chromosome condensation
Core histone N-termini play an essential role in mitotic chromosome condensation
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DOI：
10.1093/emboj/19.3.379
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发表时间：
2000-02-01
期刊：
影响因子：
11.4
通讯作者：
Dimitrov, S
中科院分区：
文献类型：
--
作者：
de la Barre, AE;Gerson, V;Dimitrov, S
我们用非洲爪哇卵提取液研究了核心组蛋白尾巴在有丝分裂染色体组装中的作用。在提取物中孵育精子核导致有丝分裂染色体的形成，我们发现这一过程与组蛋白H3在Ser10的N端尾部的磷酸化有关，当提取物中添加了III型耗竭的寡糖体时，它们不能组装染色体。通过胰酶消化选择性地消除寡体组蛋白尾巴，导致它们抑制染色体凝聚的能力急剧下降。染色体组装也被每个组蛋白尾巴以不同的效率抑制。此外，我们还发现，与拓扑异构酶II和13S凝集素不同的是，核小体通过灵活的组蛋白尾巴募集了一些染色体组装因子，这些发现证明了组蛋白尾巴在染色体组装中起着至关重要的作用。我们还提供了证据表明，核小体能够通过物理结合来耗尽SER10处的组蛋白H3磷酸化激酶的提取物，这表明该激酶可能对染色体凝聚起重要作用。
We have studied the role of core histone tails in the assembly of mitotic chromosomes using Xenopus egg extracts. Incubation of sperm nuclei in the extracts led to the formation of mitotic chromosomes, a process we found to be correlated with phosphorylation of the N-terminal tail of histone H3 at Ser10, When the extracts were supplemented with III-depleted oligosomes, they were not able to assemble chromosomes. Selective elimination of oligosome histone tails by trypsin digestion resulted in a dramatic decrease in their ability to inhibit chromosome condensation. The chromosome assembly was also inhibited by each of the histone tails with differing efficiency. In addition, we found that nucleosomes were recruiting through the flexible histone tails some chromosome assembly factors, different from topoisomerase II and 13S condensin, These findings demonstrate that histone tails play an essential role in chromosome assembly, We also present evidence that the nucleosomes, through physical association, were able to deplete the extracts from the kinase phosphorylating histone H3 at Ser10, suggesting that this kinase could be important for chromosome condensation.