Mesenchymal stromal cells: novel methods for characterization, understanding differentiation, and function.
Mesenchymal stromal cells: novel methods for characterization, understanding differentiation, and function.
复制标题
间充质基质细胞：表征、理解分化和功能的新方法。
DOI：
10.1155/2014/630936
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发表时间：
2014
影响因子：
4.3
通讯作者：
Pochampally,Radhika
中科院分区：
文献类型：
--
作者：
Tanavde,Vivek;Vemuri,MohanC;Pochampally,Radhika
MSCs are increasingly being used to treat a wide variety of disorders. These include diseases of the musculoskeletal system, wound healing, and vascular disorders. The tissue sources for MSCs are also growing. In addition to the traditional bone marrow and adipose tissue, many adult tissues have resident MSC populations that contribute to the renewal and replacement of cells in that tissue. Tissues like umbilical cord/Wharton’s jelly, dental pulp, peripheral muscle, and so forth are also gaining acceptance as viable sources of MSCs.The International Society for Cellular Therapy (ISCT) adopted a definition for MSCs in 2006. MSCs defined by these markers are not a homogenous cell population and exhibit tissue specific differences. Increasingly, MSCs are being used in a variety of veterinary applications using autologous transplantation. Therefore, there is an urgent need for complete characterization of the animal MSCs. Similar to human MSCs, animal MSCs clinical trials are hindered by lack of well-defined antibodies in different animal species for isolation and identification of those cells. Therefore, it is very important to develop new methods for MSC characterization for veterinary use. Thus, the challenges of comprehensively defining MSCs from different tissues and different species remain. The main hurdles to overcome for routine therapeutic use of MSCs have been in vitro expansion of cells (1) while retaining multipotentiality,(2) while maintaining the epigenetic and genetic stability of cells, and (3) while obtaining therapeutically effective numbers of cells efficiently. Although MSCs have been reported to differentiate into a wide variety of different cell types in vitro, it has been a challenge to generate enough differentiated cells from MSCs for therapeutic applications. This is especially true since MSCs
