Sensitive amperometric biosensor for phenolic compounds based on graphene-silk peptide/tyrosinase composite nanointerface.
Sensitive amperometric biosensor for phenolic compounds based on graphene-silk peptide/tyrosinase composite nanointerface.
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DOI：
10.1016/j.bios.2013.01.011
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发表时间：
2013-06
影响因子：
12.6
通讯作者：
Y. Qu;M. Ma;Zhengguo Wang;Guoqing Zhan;Bu-hai Li;Xian Wang;Huaifang Fang;Huijuan Zhang;Chunya Li
中科院分区：
文献类型：
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作者：
Y. Qu;M. Ma;Zhengguo Wang;Guoqing Zhan;Bu-hai Li;Xian Wang;Huaifang Fang;Huijuan Zhang;Chunya Li
制备了石墨烯-丝素肽（Gr-SP）纳米片，并与酪氨酸酶（Tyr）反应制备了一种用于酚类化合物检测的新型生物传感器。利用透射电子显微镜、X射线衍射、X射线光电子能谱、紫外/维斯和红外光谱对所制备的纳米片进行了表征。利用扫描电子显微镜和电化学阻抗谱对传感器进行了表征。以双酚A（BPA）为模型底物，考察了Gr-SP-Tyr溶液体积、外加电位、pH值、温度、Tyr/Gr-SP比例等因素对传感器分析性能的影响。该传感器对邻苯二酚、苯酚和双酚的线性响应范围分别为0.001-16.91μM、0.0015-21.12μM和2511 mAM − 1cm−2。邻苯二酚、苯酚和BPA的检测下限分别为0.23、0.35和0.72nM（S/N=3）。生物传感器还表现出良好的重复性和长期稳定性。通过对市售塑料饮料瓶中BPA浸出量的测定，验证了该传感器的实际应用。
New graphene–silk peptide (Gr–SP) nanosheets were prepared and successfully fabricated with tyrosinase (Tyr) as a novel biosensor for the determination of phenolic compounds. The Gr–SP nanosheets were fully characterized with transmission electron microscopy, x-ray diffraction, x-ray photoelectron spectroscopy, UV/Vis and FTIR spectra. The developed biosensors were also characterized with scanning electronic microscopy and electrochemical impedance spectroscopy. Using bisphenol A (BPA) as a model substrate in the sensing system, a number of key factors including the volume of Gr–SP–Tyr solution, the applied potential, pH values, temperature, and the Tyr/Gr–SP ratio that influence the analytical performance of the biosensor were investigated. The biosensor gave a linear response on the concentration ranges of 0.001–16.91μM for catechol with the sensitivity of 7634mAM−1cm−2, 0.0015–21.12μM for phenol with the sensitivity of 4082mAM−1cm−2, and 0.002–5.48μM for BPA with the sensitivity of 2511mAM−1cm−2. The low detection limits were estimated to be 0.23, 0.35 and 0.72nM (S/N=3) for catechol, phenol and BPA, respectively. The biosensors also exhibit good repeatability and long-term stability. The practical application of the biosensor was also demonstrated by the determination of BPA leaching from commercial plastic drinking bottles.