The dynamic conduct of bone marrow-derived cells in the choroidal neovascularization microenvironment
The dynamic conduct of bone marrow-derived cells in the choroidal neovascularization microenvironment
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DOI：
10.1080/02713680601100459
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发表时间：
2006-12-01
影响因子：
2
通讯作者：
Liu, Jin-Ping
中科院分区：
文献类型：
--
作者：
Hou, Hui-Yuan;Wang, Yu-Sheng;Liu, Jin-Ping
目的：脉络膜新生血管（CNV）是导致严重和进行性视力丧失的最常见原因之一。已有研究表明，骨髓源性细胞（BMCs）在CNV中发挥重要作用，表明BMCs可以成为抑制CNV发展的潜在靶点。研究CNV微环境中BMCs的动态行为有助于我们对CNV的认识。研究方法：通过将gfp+/+转基因小鼠的骨髓细胞移植到成年C57 BL/6 J小鼠体内，建立了绿色荧光蛋白（gfp）嵌合小鼠。嵌合体小鼠进行Bruch膜的激光破裂以诱导CNV，并在激光损伤后1、2、3和4周处死。摘除眼睛并进行免疫荧光处理，以检测gfp+细胞上血管平滑肌细胞（α平滑肌肌动蛋白，α SMA）、内皮细胞（CD 31）或巨噬细胞（F4/80）的标志物。通过共聚焦显微镜对所有切片进行定性和定量评估。结果：病变组织中出现大量GFP标记细胞，并整合到CNV中。在整个研究中可检测到对α SMA、CD 31或F4/80具有免疫反应性的GFP+细胞。随着CNV的发展，这三种细胞在CNV中所占的比例也发生了变化。CD 31标记细胞和F4/80标记细胞的比例在2周时达到最大值，而α SMA标记细胞的比例持续升高。结论：在CNV的发生发展过程中，BMCs的位置和表达发生了一系列变化。这些变化可能是由于BMC和CNV微环境之间的相互作用。
Purpose: Choroidal neovascularization (CNV) is one of the most frequent causes of severe and progressive vision loss. Prior studies have shown that bone marrow-derived cells (BMCs) play an important role in CNV, indicating that BMCs can be a potential target for inhibiting the development of CNV. It could be helpful for our understanding of CNV to study the dynamic conduct of BMCs in the CNV microenvironment. Methods: Green fluorescent protein (gfp) chimeric mice were developed by transplanting bone marrow cells from gfp+/+ transgenic mice to adult C57BL/6J mice. The chimeric mice underwent laser rupture of Bruch's membrane to induce CNV and were killed at 1, 2, 3, and 4 weeks after laser injury. The eyes were enucleated and processed for immunofluorescence to detect markers for vascular smooth muscle cells (alpha smooth muscle actin, alpha SMA), endothelial cells (CD31), or macrophages (F4/80) on gfp+ cells. All sections were qualitatively and quantitatively assessed by confocal microscopy. Results: Large number of gfp-labeled cells appeared in the lesions and integrated into CNV. Gfp+ cells, which were immunoreactive for alpha SMA, CD31, or F4/80, can be detected through the whole study. The constituent ratio of those three cell-types in total gfp+ cells in CNV altered as CNV developed. The maximal ratios of CD31-labeled cells and F4/80-labeled cells presented at 2 week, while the ratio of alpha SMA-labeled cells upgraded continuously. Conclusions: BMCs underwent a serial of changes in position and expression during the progression of CNV. Those changes may result from the interaction between BMCs and the CNV microenvironment.