An improved radioimmunoassay of triiodothyronine in serum: its application to clinical and physiological studies.
An improved radioimmunoassay of triiodothyronine in serum: its application to clinical and physiological studies.
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血清中三碘甲状腺原氨酸的改进放射免疫测定法：其在临床和生理研究中的应用。
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发表时间：
1972
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通讯作者：
Robert W. Lam
中科院分区：
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作者：
Inder J. Chopra;Ruey S. Ho;Robert W. Lam
本文介绍了一种灵敏、特异、简便、重复性好的放射免疫分析法（RIA）测定未提取血清中三碘甲状腺原氨酸（T3）。RIA的特点是用切除甲状腺的羊血清作标准曲线，用8-苯胺基-1-萘磺酸（ANS）抑制T3与甲状腺结合球蛋白（TBG）的结合。96例甲状腺功能正常者血清T3浓度为112.8 ± 3.3ng.每100 ml。(mean± S.E.M.）;为490.7 ± 42.3ng。每100 ml。30例甲亢患者为40.1 ± 7.6ng.每100 ml。12例甲减患者为157.6 ± 31.2ng。每100 ml。10例甲状腺功能正常的血清TBG升高的受试者。4名正常受试者对肌肉注射TSH（Thytropar，10 I.U.）血清T3在4 - 6小时最大增加75 - 195%（平均130），这些患者血清T4最大增加36.4 - 92.7%（平均57.2），发生在TSH给药后12 - 24小时。7名甲状腺功能正常的受试者，静脉注射200 μg促甲状腺激素释放激素（TRH）后，血清T3在2 ~ 4小时最大增加29%~ 115%（平均72）;血清T4最大增加也发生在TRH给药后2 ~ 4小时，但增幅较小，为13%~ 75%（平均37）。
Abstract A sensitive, specific, convenient, and reproducible radioimmunoassay (RIA) for measurement of triiodothyronine (T 3 ) in unextracted serum is described. The features of the RIA which permit testing of unextracted serum include the use of a thyroidectomized sheep serum for construction of the standard curve and of 8-anilino-1-naphthalene sulfonic acid (ANS) to inhibit binding of T 3 to thyroxinebinding globulin (TBG). The serum T 3 concentration in 96 euthyroid subjects was 112.8 ± 3.3 ng. per 100 ml. (mean ± S.E.M.); it was 490.7 ± 42.3 ng. per 100 ml. in 30 hyperthyroid patients, 40.1 ± 7.6 ng. per 100 ml. in 12 hypothyroid patients, and 157.6 ± 31.2 ng. per 100 ml. in 10 euthyroid subjects with elevated serum TBG. Four normal subjects responded to intramuscular administration of TSH (Thytropar, 10 I.U.) with a maximal increase in serum T 3 of 75 to 195 per cent (mean 130) at 4 to 6 hours; the maximal increase in serum T 4 in these patients ranged between 36.4 and 92.7 per cent (mean 57.2), and occurred 12 to 24 hours after TSH administration. Seven euthyroid subjects, given 200 μg of thyrotropin-releasing hormone (TRH) intravenously, demonstrated a maximal increase in serum T 3 of 29 to 115 per cent (mean 72) at 2 to 4 hours; the maximal increase in serum T 4 also occurred at 2 to 4 hours of TRH administration but was more modest, 13 to 75 per cent (mean 37).