ISOLATION OF THE AVIAN TRANSFORMING RETROVIRUS, AS42, CARRYING THE V-MAF ONCOGENE AND INITIAL CHARACTERIZATION OF ITS GENE-PRODUCT
ISOLATION OF THE AVIAN TRANSFORMING RETROVIRUS, AS42, CARRYING THE V-MAF ONCOGENE AND INITIAL CHARACTERIZATION OF ITS GENE-PRODUCT
复制标题
DOI：
10.1016/0042-6822(92)90532-t
复制
发表时间：
1992-06-01
期刊：
影响因子：
3.7
通讯作者：
NISHIZAWA, M
中科院分区：
文献类型：
--
作者：
KAWAI, S;GOTO, N;NISHIZAWA, M
从鸡肌肉腱膜纤维肉瘤中分离到一种新的禽转化逆转录病毒。这种病毒（称为AS 42）诱导肿瘤组织病理学上无法区分从原来的肉瘤后，很长一段潜伏期接种到新生鸡。AS 42在感染鸡胚成纤维细胞（CEF）时也表现出弱的转化活性。这种病毒是复制缺陷型的，与A亚群的辅助病毒（称为ASAV）有关。AS 42特异性蛋白质的约100 kDa的免疫沉淀从AS 42转化CEF裂解物与抗禽逆转录病毒病毒粒子蛋白的抗血清。对AS 42病毒基因组结构的分子分析已经揭示，这种100-kDa蛋白质代表了一种新的致癌基因，细胞来源的v-maf，其与病毒抗原基因的一部分融合（Nishizawaet al.，Proc. Natl. Acad. Sci. USA86，7711- 7715，1989）。有趣的是，在转化的CEF的单个克隆中发现的gag-mafflasts蛋白之间观察到一些大小变化。与这一观察结果一致，Southern印迹分析和核苷酸序列测定的几个独立的分离株的前病毒DNA表明，这种病毒分离多种形式的缺失突变体，可能是通过同源重组之间的重复序列存在于v-maf编码区。
A novel avian transforming retrovirus was isolated from a chicken musculoaponeurotic fibrosarcoma. This virus (called AS42) induces tumors histopathologically indistinguishable from the original sarcoma after a long latent period when inoculated into newborn chickens. AS42 also exhibits a weak transforming activity when infected into chicken embryo fibroblasts (CEF). This virus is replication-defective and associated with a helper virus of subgroup A (called ASAV). An AS42-specific protein of about 100 kDa was immunoprecipitated from lysates of AS42-transformed CEF with antiserum directed against avian retrovirus virion proteins. Molecular analysis of the genomic structure of the AS42 virus has revealed that this 100-kDa protein represents a novel oncogene, v-mafof cellular origin, which is fused with a part of the viralgaggene (Nishizawaet al., Proc. Natl. Acad. Sci. USA86, 7711–7715,1989). Interestingly, some size variation was observed among the gag-maffusion proteins found in individual clones of transformed CEF. Consistent with this observation, Southern blot analyses and nucleotide sequence determination of several independent isolates of proviral DNA indicated that this virus segregates multiple forms of deletion mutants, probably through homologous recombinations among the repetitive sequences present within the v-maf coding region.