Effects of eNOS rs1799983 and ACE rs4646994 polymorphisms on the therapeutic efficacy of salvianolate injection in Chinese patients with coronary heart disease
Effects of eNOS rs1799983 and ACE rs4646994 polymorphisms on the therapeutic efficacy of salvianolate injection in Chinese patients with coronary heart disease
复制标题
DOI：
10.1111/1440-1681.12257
复制
发表时间：
2014-08-01
影响因子：
2.9
通讯作者：
Liu, Zhao-Qian
中科院分区：
文献类型：
--
作者：
Fang, Chao;Ren, Xian;Liu, Zhao-Qian
本研究旨在阐明eNOS 894G/T和ACE I/D基因多态性与冠心病（CHD）风险的关系，并探讨这些基因多态性对丹参酚酸注射液治疗中国冠心病患者疗效的影响。总共有153名冠心病患者和198名健康对照者参加了这项研究。采集外周血5ml进行DNA提取。采用直接测序法确定eNOS 894G/T的遗传诊断。采用聚合酶链反应-限制性片段长度多态性分析检测ACE I/D基因型。我们观察到冠心病患者与健康对照组eNOS和ACE多态性的频率分布差异有统计学意义（P < 0.05）。二元logistic逐步回归分析显示，基因型对患者的治疗效果有加性和显性影响（P < 0.05）。这些数据提示ACE I/D和eNOS 894G/T基因多态性可能在冠心病的发生发展中发挥作用，这些基因多态性可能影响中国冠心病患者对丹参酚酸注射液的反应。
The purpose of the present study was to clarify the association of eNOS 894G/T and ACE I/D genetic polymorphisms with the risk of coronary heart disease (CHD) and to explore the effects of these polymorphisms on the therapeutic efficacy of salvianolate injection in Chinese CHD patients. In all, 153 CHD patients and 198 healthy controls were enrolled in the study. We collected 5 mL peripheral blood for DNA extraction. Genetic diagnosis of eNOS 894G/T was determined by direct sequencing. Polymerase chain reaction-restriction fragment length polymorphism analysis was used to detect ACE I/D genotypes. We observed significant differences in the frequency distribution of eNOS and ACE polymorphisms between CHD patients and healthy controls (P < 0.05). Binary logistic regression stepwise analysis revealed that the genotypes had an additive and dominant effect on patients' therapeutic responses (P < 0.05). These data suggest that the genetic polymorphisms of ACE I/D and eNOS 894G/T probably play a role in the development of CHD and these genetic polymorphisms may affect the response to salvianolate injection in Chinese CHD patients.