Adipocyte differentiation‐related protein reduces lipid droplet association of adipose triglyceride lipase and slows triacylglycerol turnover
Adipocyte differentiation‐related protein reduces lipid droplet association of adipose triglyceride lipase and slows triacylglycerol turnover
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DOI：
10.1096/fasebj.21.5.a235-a
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发表时间：
2007-04
期刊：
影响因子：
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通讯作者：
L. Listenberger;Elysa B. Goldberg;William J Brown;Deborah A Brown
中科院分区：
文献类型：
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作者：
L. Listenberger;Elysa B. Goldberg;William J Brown;Deborah A Brown
尽管中性脂肪储存液滴无处不在，它们在肥胖中的重要性，但人们对它们的合成和周转是如何控制的知之甚少。调节这些过程的一个主要候选蛋白是脂肪细胞分化相关蛋白(adrp；也称为脂肪蛋白)。为了确定adrp影响脂质储存的机制，我们在HEK 293细胞中稳定表达了该蛋白。ADRP定位于293细胞的脂滴表面，并在未添加和添加油酸盐的条件下增加了三酰甘油(TAG)的质量。至少部分肿块的增加是由于ADRP表达细胞中Tag的水解率降低了2倍。此外，adrp的表达导致脂肪甘油三酯脂肪酶(ATGL)与脂滴的关联性显著降低。ATGL和/或其他脂肪酶的脂滴结合减少可能解释了在ADRP表达细胞中观察到的标签周转减少的原因。
Despite the ubiquity of neutral lipid storage droplets and their importance in obesity, very little is known about how their synthesis and turnover are controlled. A major candidate for regulating these processes is adipocyte differentiation‐related protein (ADRP; also known as adipophilin). To determine the mechanism through which ADRP affects lipid storage, we stably expressed the protein in HEK 293 cells. ADRP was targeted to the surface of lipid droplets in 293 cells and increased triacylglycerol (TAG) mass under both unsupplemented and oleate‐supplemented conditions. At least part of the increased mass resulted from a 2‐fold decrease in the rate of TAG hydrolysis in ADRP‐expressing cells. Furthermore, ADRP expression induced a significant decrease in association of adipose triglyceride lipase (ATGL) with lipid droplets. Reduced lipid droplet association of ATGL and/or other lipases may explain the decrease in TAG turnover observed in ADRP‐expressing cells.