FGF signaling controls somite boundary position and regulates segmentation clock control of spatiotemporal Hox gene activation
FGF signaling controls somite boundary position and regulates segmentation clock control of spatiotemporal Hox gene activation
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DOI：
10.1016/s0092-8674(01)00437-8
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发表时间：
2001-07-27
期刊：
影响因子：
64.5
通讯作者：
Pourquié, O
中科院分区：
文献类型：
--
作者：
Dubrulle, J;McGrew, MJ;Pourquié, O
脊椎动物的分割需要一个分子振荡器，即分割时钟，作用于分裂前中胚层(PSM)细胞来设定划分节段边界的速度。然而，定位每条边界的信号仍不清楚。在这里，我们报告，在后方的PSM中表达的FGF8产生了一个移动的波前，在该波前水平上，分段边界位置和轴向一致性都被确定。此外，通过操纵鸡胚的边界位置，我们表明HOX基因的表达在适当编号的体节中保持不变，而不是在绝对的轴向位置。这些结果表明，FGF8确保了分割过程和时空HOX基因激活的紧密协调。
Vertebrate segmentation requires a molecular oscillator, the segmentation clock, acting in presomitic mesoderm (PSM) cells to set the pace at which segmental boundaries are laid down. However, the signals that position each boundary remain unclear. Here, we report that FGF8 which is expressed in the posterior PSM, generates a moving wavefront at which level both segment boundary position and axial identity become determined. Furthermore, by manipulating boundary position in the chick embryo, we show that Hox gene expression is maintained in the appropriately numbered somite rather than at an absolute axial position. These results implicate FGF8 in ensuring tight coordination of the segmentation process and spatiotemporal Hox gene activation.