NOISE EXPOSURE ALTERS THE RESPONSE OF OUTER HAIR-CELLS TO ATP
NOISE EXPOSURE ALTERS THE RESPONSE OF OUTER HAIR-CELLS TO ATP
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DOI：
10.1016/0378-5955(95)00115-k
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发表时间：
1995-08-01
期刊：
影响因子：
2.8
通讯作者：
BOBBIN, RP
中科院分区：
文献类型：
--
作者：
CHEN, C;NENOV, A;BOBBIN, RP
外毛细胞（OHC）是噪声诱导效应的靶细胞之一。到目前为止，很少有研究探讨的OHC的功能变化引起的噪声暴露。越来越多的证据表明ATP可能是一种作用于OHC的神经调质。因此，我们研究了低水平噪声暴露可能改变对ATP的反应的可能性。ATP的阳离子电流进行了测试记录从外毛细胞（OHC）从慢性噪声暴露的豚鼠，并从正常对照动物记录的电流进行比较。采用全细胞膜片钳技术。当采用正常的内部和外部溶液时，与对照组相比，噪声暴露动物的OHC对100 μ M ATP的反应发生率降低。当移液器溶液中的K+被N-甲基-葡糖胺（NMG（+））取代时，噪声暴露和对照动物的细胞之间的ATP诱发电流的幅度存在显著差异。这在正常和20 mM Ba 2+外部溶液中均观察到。此外，对ATP的反应表现出依赖于OHC长度。简言之，暴露于噪声的动物的OHC（< 65 μ m）对ATP的反应幅度显著降低。相比之下，暴露于噪声的动物的长OHC（> 65 μ m）的反应增加。结果表明，低水平的噪声暴露诱导OHC的变化，影响细胞对ATP的反应。
The outer hair cells (OHCs) are one target of noise-induced effects. To date there are few studies which examine changes in the function of OHCs induced by noise exposure. There is increasing evidence that ATP may be a neuromodulator acting on OHCs. Therefore, we examined the possibility that the response to ATP may be altered by low-level noise exposure. ATP was tested on cation currents recorded from outer hair cells (OHCs) isolated from chronic noise-exposed guinea pigs and compared to currents recorded from normal control animals. The whole-cell variant of the patch-clamp technique was used. The incidence of response to 100 mu M ATP was decreased in OHCs from noise-exposed animals as compared to controls when normal internal and external solutions were employed. When K+ was substituted by N-methyl-glucamine (NMG(+)) in the pipette solution, there were significant differences in the magnitudes of ATP-evoked currents between cells from noise-exposed and control animals. This was observed in both normal and 20 mM Ba2+ external solutions. In addition, the response to ATP exhibited a dependency on OHC length. In short OHCs (< 65 mu m) from noise-exposed animals the magnitude of the response to ATP was significantly reduced. By contrast, the response in long OHCs (> 65 mu m) from noise-exposed animals was increased. Results suggest that low-level noise exposure induces changes in OHCs which affect the response of the cell to ATP.