Spectra of Some Amino Acids, Peptides, Nucleic Acids, and Protein in the Vacuum Ultraviolet

Spectra of Some Amino Acids, Peptides, Nucleic Acids, and Protein in the Vacuum Ultraviolet
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某些氨基酸、肽、核酸和蛋白质的真空紫外光谱

DOI:
10.1063/1.1742806
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发表时间:
1956
影响因子:
4.4
通讯作者:
R. Setlow
R. Setlow
中科院分区:
化学2区
文献类型:
--
作者:
J. Preiss;R. Setlow

文献摘要

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已经获得了几种氨基酸、肽、核酸和一种蛋白质的吸收光谱,其吸收范围低至1450 A。样品是以薄膜的形式支撑在氟化钙上。所有化合物在2000 A以下均有特征吸收光谱。在一些情况下,色氨酸,固体膜的吸收光谱与溶液中分子的光谱显示出明显的差异。在1900 A,蛋白质糜蛋白酶的吸收系数是其组分的分子消光系数之和。使用的仪器是具有氢放电光源的真空光栅单色仪。
The absorption spectra of several amino acids, peptides, nucleic acids, and a protein have been obtained down to 1450 A. The samples were in the form of thin films supported on calcium fluoride. All compounds studied had characteristic absorption spectra below 2000 A. In some cases, e.g., tryptophan, the absorption spectrum of the solid film showed pronounced difference from the spectrum of the molecules in solution. At 1900 A, the absorption coefficient of the protein chymotrypsin is the sum of the molecular extinction coefficients of its components. The instrument used was a vacuum grating‐monochromator with hydrogen discharge light source.