Probing the initiation and effector phases of the somatic piRNA pathway in Drosophila
Probing the initiation and effector phases of the somatic piRNA pathway in Drosophila
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DOI：
10.1101/gad.1968110
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发表时间：
2010-11-15
影响因子：
10.5
通讯作者：
Hannon, Gregory J.
中科院分区：
文献类型：
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作者：
Haase, Astrid D.;Fenoglio, Silvia;Hannon, Gregory J.
结合培养细胞中的RNAi和突变动物的分析，我们探索了已知的Piwi相互作用RNA（皮尔纳）途径组分在转座子沉默的起始和效应阶段中的作用。南瓜与Piwi物理相关，其表达的减少导致适度的转座子去阻遏，而对piRNA没有影响，这与效应子作用一致。西葫芦或Armitage的改变减少了Piwi蛋白和piRNA，表明在形成稳定的Piwi RISC（RNA诱导的沉默复合物）中的功能。值得注意的是，西葫芦的丢失或其催化结构域内的突变导致来自弗拉门戈的未加工的前体转录物的积累，这与这种推定的核酸酶在皮尔纳生物发生中的作用一致。
Combining RNAi in cultured cells and analysis of mutant animals, we probed the roles of known Piwi-interacting RNA (piRNA) pathway components in the initiation and effector phases of transposon silencing. Squash associated physically with Piwi, and reductions in its expression led to modest transposon derepression without effects on piRNAs, consistent with an effector role. Alterations in Zucchini or Armitage reduced both Piwi protein and piRNAs, indicating functions in the formation of a stable Piwi RISC (RNA-induced silencing complex). Notably, loss of Zucchini or mutations within its catalytic domain led to accumulation of unprocessed precursor transcripts from flamenco, consistent with a role for this putative nuclease in piRNA biogenesis.