Synthesis and characterization of pHLIP(®) coated gold nanoparticles.
Synthesis and characterization of pHLIP(®) coated gold nanoparticles.
复制标题
DOI：
10.1016/j.bbrep.2017.02.008
复制
发表时间：
2017-07
影响因子：
2.7
通讯作者：
Reshetnyak YK
中科院分区：
文献类型：
--
作者：
Daniels JL;Crawford TM;Andreev OA;Reshetnyak YK
介绍了聚乙二醇（PEG）和pH低插入肽（pHLIP®）包裹的球形和多钉状金纳米粒子的合成新方法。纳米颗粒涂层中肿瘤靶向pHLIP®肽的存在增强了颗粒在溶液中的稳定性，并促进了pH依赖性细胞摄取。用柠檬酸钠作为金还原剂制备球形颗粒，以形成平均金属核直径为7.0±2.5 nm和平均流体动力学直径为1.43 nm的颗粒。注射到小鼠肿瘤中的颗粒（21 µg金）均匀分布在肿瘤块内，肿瘤附近的肌肉组织没有染色。注射后1小时，高达30%的注射金剂量保留在肿瘤内。以抗坏血酸为还原剂，圆盘状双胞为模板，制备了平均粒径为146.0±50.4 nm，平均流体力学粒径为161 nm的多钉状金纳米粒子。只有软模板的存在，如bicelles，确保了在近红外区共振的钉状纳米粒子的外观。用805 nm的激光照射掺金纳米粒子，导致温度随时间和浓度的增加而增加。pHLIP®和PEG涂层的金球形和多刺纳米颗粒都可能在肿瘤的放射和热疗法中找到应用。合成pHLIP®-PEG涂覆的pH敏感性金球形纳米颗粒。注射后1小时，注射的金剂量的30%保留在肿瘤内。合成了pHLIP®-PEG包被的pH敏感性金多刺纳米颗粒。双胞被用作软模板，以获得多掺纳米粒子。在805 nm照射掺金纳米颗粒后，温度升高。
Novel approaches in synthesis of spherical and multispiked gold nanoparticles coated with polyethylene glycol (PEG) and pH Low Insertion Peptide (pHLIP®) were introduced. The presence of a tumor-targeting pHLIP® peptide in the nanoparticle coating enhances the stability of particles in solution and promotes a pH-dependent cellular uptake. The spherical particles were prepared with sodium citrate as a gold reducing agent to form particles of 7.0±2.5 nm in mean metallic core diameter and ∼43 nm in mean hydrodynamic diameter. The particles that were injected into tumors in mice (21 µg of gold) were homogeneously distributed within a tumor mass with no staining of the muscle tissue adjacent to the tumor. Up to 30% of the injected gold dose remained within the tumor one hour post-injection. The multispiked gold nanoparticles with a mean metallic core diameter of 146.0±50.4 nm and a mean hydrodynamic size of ~161 nm were prepared using ascorbic acid as a reducing agent and disk-like bicelles as a template. Only the presence of a soft template, like bicelles, ensured the appearance of spiked nanoparticles with resonance in the near infrared region. The irradiation of spiked gold nanoparticles by an 805 nm laser led to the time- and concentration-dependent increase of temperature. Both pHLIP® and PEG coated gold spherical and multispiked nanoparticles might find application in radiation and thermal therapies of tumors. pHLIP®-PEG coated pH-sensitive gold spherical nanoparticles were synthesized. 30% of the injected gold dose remained within the tumor one hour post-injection. pHLIP®-PEG coated pH-sensitive gold multispiked nanoparticles were synthesized. Bicelles were used as a soft template to obtain multispiked nanoparticles. Temperature increases after 805 nm irradiation of spiked gold nanoparticles.