OCCURRENCE OF THIOL PROTEINASES IN THE EGGS OF THE SILKWORM, BOMBYX-MORI
OCCURRENCE OF THIOL PROTEINASES IN THE EGGS OF THE SILKWORM, BOMBYX-MORI
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DOI：
10.1093/oxfordjournals.jbchem.a133521
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发表时间：
1981-01-01
影响因子：
2.7
通讯作者：
TAKAHASHI, K
中科院分区：
文献类型：
--
作者：
KAGEYAMA, T;TAKAHASHI, SY;TAKAHASHI, K
家蚕(B.mori)成熟卵粗提物中，蛋白水解酶活性仅在酸性pH区检测到，在pH 3.5时活性最大，这种活性在整个发育过程中一直保持。无论是成熟卵还是胚胎早期的卵，在中性到碱性的pH区都没有观察到明显的活性。从成熟的鸡蛋中获得了两种在pH 3.5下活性的蛋白酶分子形式，它们的相对分子质量分别为>；160,000和.apprx。分别为6.8万人。另一种4万兆瓦的形式出现在孵化前的卵子中。对氯化汞苯甲酸酯、对氯化汞苯磺酸盐和N-[N-(L-3-trans-carboxyoxirane-2-carbonyl)-L-leucyl]agmatine(E-)对这些酶有较强的抑制作用，而胃抑素、二异丙基氟磷酸盐和乙二胺四乙酸二钠对其无抑制作用，提示它们是硫醇酶。它们能降解酪蛋白、牛血清白蛋白、卵白蛋白和丙种球蛋白。它们不能水解α-N-苯甲酰基-DL-精氨酸对硝基苯胺、α-N-苯甲酰基-DL-精氨酸β-萘胺和其他几种合成底物。这些硫醇蛋白水解酶不同于迄今已知的动物组织硫醇蛋白水解酶。
In the crude extract of matured eggs of the silkworm (B. mori), proteolytic activity was detected only in the acidic pH region with maximal activity at pH 3.5; this activity was maintained throughout development. No appreciable activity was observed in the neutral to alkaline pH region either in matured eggs or in eggs at early embryonic stages. Two molecular forms of proteinases active at pH 3.5 were obtained from matured eggs, and their MW were estimated to be > 160,000 and .apprx. 68,000, respectively. An additional form of 40,000 MW appeared in eggs just before hatching. They were strongly inhibited by thiol proteinase inhibitors, such as p-chloromercuribenzoate (pCMB), p-chloromercuriphenyl sulfonate (pCMPS) and N-[N-(L-3-trans-carboxyoxirane-2-carbonyl)-L-leucyl]agmatine (E-64); pepstatin, diisopropylphosphorofluoridate, and EDTA were without effect, suggesting that they are thiol enzymes. They hydrolyzed casein, bovine serum albumin, egg albumin and .gamma.-globulin. They could not hydrolyze .alpha.-N-benzoyl-DL-arginine p-nitroanilide, .alpha.-N-benzoyl-DL-arginine .beta.-naphthylamide and several other synthetic substrates. These thiol proteinases differ from hitherto known animal tissue thiol proteinases.