RECONSTITUTION OF EMULSIFYING ACTIVITY OF ACINETOBACTER-CALCOACETICUS BD4 EMULSAN BY USING PURE POLYSACCHARIDE AND PROTEIN
RECONSTITUTION OF EMULSIFYING ACTIVITY OF ACINETOBACTER-CALCOACETICUS BD4 EMULSAN BY USING PURE POLYSACCHARIDE AND PROTEIN
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DOI：
10.1128/aem.53.2.440-446.1987
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发表时间：
1987-02-01
影响因子：
4.4
通讯作者：
ROSENBERG, E
中科院分区：
文献类型：
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作者：
KAPLAN, N;ZOSIM, Z;ROSENBERG, E
醋酸钙不动杆菌BD4和BD413在2%乙醇培养基上生长时产生胞外乳化剂。对于乳化活性，多糖和蛋白质组分都是必需的，如通过用特定的噬菌体携带的多糖解聚酶选择性消化多糖、用热苯酚使胞外乳化复合物脱蛋白质以及用纯多糖和无多糖蛋白质组分重建乳化剂活性所证明的。多糖中羧基的化学修饰导致活性损失。将重建乳化活性所需的蛋白质纯化七倍。BD 4 emulsan的两亲性显然来自于阴离子亲水性多糖与蛋白质的缔合。
Acinetobacter calcoaceticus BD4 and BD413 produce extracellular emulsifying agents when grown on 2% ethanol medium. For emulsifying activity, both polysaccharide and protein fractions were required, as demonstrated by selective digestion of the polysaccharide with a specific bacteriophage-borne polysaccharide depolymerase, deproteinization of the extracellular emulsifying complex with hot phenol, and reconstitution of emulsifier activity with pure polysaccharide and a polysaccharide-free protein fraction. Chemical modification of the carboxyl groups in the polysaccharide resulted in a loss of activity. The protein required for reconstitution of emulsifying activity was purified sevenfold. The BD4 emulsan apparently derives it''s amphipathic properties from the association of an anionic hydrophilic polysaccharide with proteins.