Comparative studies on tryptophan binding to hepatic nuclear envelopes in Sprague-Dawley and Lewis rats.
Comparative studies on tryptophan binding to hepatic nuclear envelopes in Sprague-Dawley and Lewis rats.
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Sprague-Dawley 和 Lewis 大鼠色氨酸与肝核包膜结合的比较研究。
DOI：
10.1152/ajpregu.1994.267.2.r502
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Verney,E
中科院分区：
文献类型：
--
作者：
Sidransky,H;Verney,E
由于刘易斯大鼠易患许多炎性疾病，并已被用于嗜酸性粒细胞增多症-肌痛综合征的实验模型，我们研究了刘易斯大鼠是否会响应L-色氨酸，如先前报道的Sprague-Dawley大鼠。在本比较研究中，使用两种品系的雌性大鼠，我们观察到与Sprague-Dawley大鼠相比，刘易斯大鼠体外L-色氨酸与肝细胞核和核膜结合的亲和力降低。然而，在体内的刺激作用，管理L-色氨酸对肝多聚核糖体聚集，蛋白质合成，和核RNA的释放是相似的两种菌株。在体外[3 H]色氨酸结合到肝核膜，使用L-色氨酸牵连嗜酸性粒细胞增多症-肌痛综合征的情况下，显示较少的特异性结合比使用noninvolved L-色氨酸在两种菌株。与Sprague-Dawley大鼠相比，L-色氨酸与刘易斯大鼠肝核结合亲和力的定量差异的可能意义尚未确定。
Since Lewis rats are susceptible to many inflammatory diseases and have been used in an experimental model of the eosinophilia-myalgia syndrome, we investigated whether Lewis rats would respond to L-tryptophan as have Sprague-Dawley rats reported earlier. In this comparative study using females of both strains, we observed a decrease in the affinity of in vitro L-tryptophan binding to hepatic nuclei and nuclear envelopes of Lewis rats compared with Sprague-Dawley rats. However, in vivo stimulatory effects of administering L-tryptophan on hepatic polyribosomal aggregation, protein synthesis, and nuclear RNA release were similar in both strains. In vitro [3H]tryptophan binding to hepatic nuclear envelopes, using L-tryptophan implicated in cases of the eosinophilia-myalgia syndrome, revealed less specific binding than when using nonimplicated L-tryptophan in both strains. The possible significance of the quantitative difference in the binding affinity of L-tryptophan to hepatic nuclei of Lewis rats compared with those of Sprague-Dawley rats is as yet undetermined.