Mononuclear cells from human lung parenchyma support antigen-induced T lymphocyte proliferation.
Mononuclear cells from human lung parenchyma support antigen-induced T lymphocyte proliferation.
复制标题
来自人肺实质的单核细胞支持抗原诱导的 T 淋巴细胞增殖。
DOI：
10.1002/jlb.45.4.336
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发表时间：
1989
影响因子：
5.5
通讯作者：
Toews,GB
中科院分区：
文献类型：
--
作者：
Nicod,LP;Lipscomb,MF;Weissler,JC;Toews,GB
我们之前已经证明，有一个松散贴壁的肺单核细胞亚群，可以从切碎和酶消化的肺组织中分离出来，具有刺激同种异体 T 淋巴细胞增殖的强大能力。我们现在证明这些细胞还能够刺激自体混合白细胞反应 (AMLR) 并向自体 T 淋巴细胞呈递抗原。这些松散粘附的单核细胞（LAM）作为抗原呈递细胞比肺泡巨噬细胞或单核细胞更有效。 LAM 群体中吞噬细胞或 Fc 受体阳性细胞的消耗增强了 AMLR 反应的刺激，同时保留了抗原诱导的 T 淋巴细胞增殖。这些结果表明，人肺实质中存在非吞噬性 Fc 受体阴性辅助细胞，能够激活 AMLR 中的静息 T 细胞并支持抗原特异性 T 淋巴细胞增殖。这些细胞的身份尚不确定，但数据强烈表明该细胞不是经典的单核细胞衍生的巨噬细胞。这些抗原呈递细胞可能对于肺内免疫反应的启动至关重要。
We have previously demonstrated that there is a subpopulation of loosely adherent pulmonary mononuclear cells that can be isolated from minced and enzyme‐digested lung tissue with a potent capacity to stimulate allogeneic T lymphocyte proliferation. We now demonstrate that these cells are also capable of stimulating an autologous mixed leukocyte reaction (AMLR) and presenting antigen to autologous T lymphocytes. These loosely adherent mononuclear cells (LAM) were more effective than either alveolar macrophages or monocytes as antigen‐presenting cells. Depletion of phagocytic or Fc receptor‐positive cells from the LAM population enhanced the stimulation of an reaction AMLR while preserving antigen‐induced T lymphocyte proliferation. These results indicate that there are nonphagocytic, Fc receptor‐negative accessory cells in human lung parenchyma capable of activating resting T cells in an AMLR and supporting antigen‐specific T lymphocyte proliferation. The identity of these cells is uncertain, but the data strongly suggest that the cell is not a classical monocyte‐derived macrophage. These antigen‐presenting cells may be critical in the initiation of immune responses within the lung.