The medial prefrontal cortex differentially regulates stress-induced c-fos expression in the forebrain depending on type of stressor
The medial prefrontal cortex differentially regulates stress-induced c-fos expression in the forebrain depending on type of stressor
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DOI：
10.1046/j.1460-9568.2003.02932.x
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发表时间：
2003-10-01
影响因子：
3.4
通讯作者：
Herman, JP
中科院分区：
文献类型：
--
作者：
Figueiredo, HF;Bruestle, A;Herman, JP
内侧前额叶皮质（mPFC）在下丘脑-垂体-肾上腺（HPA）轴反应中起重要的抑制作用。参与的mPFC似乎取决于类型的压力，优先影响“心因性”刺激。在这项研究中，我们映射的c-fos mRNA的表达，以评估神经回路的应激特异性行动的mPFC HPA反应。因此，mPFC损伤和假手术大鼠组被限制为20分钟或暴露于乙醚烟雾2分钟。在这两种情况下，动物被杀死在40分钟的应激发作。有趣的是，双侧病变的mPFC显着增强c-fos mRNA的表达在下丘脑室旁核的限制动物，这种效果是通过增强循环ACTH浓度在这些动物中。另一方面，病变的mPFC不影响PVN c-fos mRNA的表达，也不影响血浆ACTH浓度暴露于乙醚的动物。mPFC的病变也增强了内侧杏仁核的c-fos激活后的约束，但不乙醚暴露。其活动受到mPFC病变或应激差异影响的其他区域包括终纹床核的腹外侧部、CA 3海马、梨状皮质和背侧梨状核。所有应激动物的中央杏仁核几乎不表达c-fos mRNA，无论病变。此外，前额叶皮质病变并没有改变CA 1海马，齿状回，前内侧分裂的终纹，外侧隔，屏状核的床核的c-fos的应激诱导水平。两者合计，这项研究表明，内侧前额叶皮层差异调节特定的压力相关的大脑区域的细胞激活，从而施加压力依赖性抑制HPA轴。
The medial prefrontal cortex (mPFC) plays an important inhibitory role in the hypothalamic-pituitary-adrenal (HPA) axis response. The involvement of the mPFC appears to depend on the type of stressor, preferentially affecting 'psychogenic' stimuli. In this study, we mapped expression of c-fos mRNA to assess the neural circuitry underlying stressor-specific actions of the mPFC on HPA reactivity. Thus, groups of mPFC-lesioned and sham-operated rats were restrained for 20 min or exposed to ether fumes for 2 min. In both cases, the animals were killed at 40 min from the onset of stress. Interestingly, bilateral lesions of the mPFC significantly enhanced c-fos mRNA expression in the hypothalamic paraventricular nucleus of restrained animals, an effect that was paralleled by potentiation of circulating ACTH concentrations in these animals. On the other hand, lesions of the mPFC did not affect neither PVN c-fos mRNA expression nor plasma ACTH concentrations in animals exposed to ether. Lesions of the mPFC also enhanced c-fos activation in the medial amygdala following restraint, but not following ether exposure. Additional regions whose activity was affected by mPFC lesions or stressor differences included the ventrolateral division of the bed nucleus of the stria terminalis, CA3 hippocampus, piriform cortex, and dorsal endopiriform nucleus. Expression of c-fos mRNA was nearly absent in the central amygdala of all stressed animals, regardless of lesion. Furthermore, prefrontal cortex lesions did not change stress-induction levels of c-fos in the CA1 hippocampus, dentate gyrus, anteromedial division of the bed nucleus of the stria terminalis, lateral septum, and claustrum. Taken together, this study indicates that the medial prefrontal cortex differentially regulates cellular activation of specific stress-related brain regions, thus exerting stressor-dependent inhibition of the HPA axis.