Quick and In Situ Detection of Different Polar Allelochemicals in Taxus Soil by Microdialysis Combined with UPLC-MS/MS
Quick and In Situ Detection of Different Polar Allelochemicals in Taxus Soil by Microdialysis Combined with UPLC-MS/MS
复制标题
微透析结合 UPLC-MS/MS 快速原位检测红豆杉土壤中不同极性化感物质
DOI：
10.1021/acs.jafc.2c06912
复制
发表时间：
2022
影响因子：
6.1
通讯作者：
Chunjian Zhao
中科院分区：
文献类型：
--
作者：
Bin Qiao;Siming Nie;Qianqian Li;Zahid Majeed;Jiabo Cheng;Zhanyu Yuan;Chunying Li;Chunjian Zhao
化感作用的发挥需要土壤中存在化感物质并达到一定浓度。此外，土壤中化感物质的检测也是化感作用探索过程中最重要的研究课题之一。为了解决低浓度、不同极性的化感物质同时检测的问题，本研究首次在不破坏土壤原有结构的基础上，建立了微渗析-UPLC-MS/MS联用技术快速检测红豆杉土壤化感物质的新方法。最佳透析条件为：甲醇浓度70%，L流量3μ/min，膜管长度3 cm。系统优化了一套可靠的UPLC-MS/MS联用程序，同时检测了9种不同极性的化感物质。结果表明，9种化感物质在三种不同红豆杉土壤中的含量和分布存在差异。
The action of allelopathy need that allelochemicals exist in the soil and reach a certain concentration. Also, the detection of allelochemicals in the soil is one of the most important research topics in the process of exploring allelopathy. To solve the problem of the simultaneous detection of allelochemicals with low concentrations and different polarities, a novel strategy for the quick detection of the allelochemicals inTaxussoil by microdialysis combined with UPLC-MS/MS on the basis ofin situdetection without destroying the original structure of soil was developed for the first time in the work. The dialysis conditions were optimized by the Box–Behnken design (BBD): 70% methanol, 3 μL/min flow rate, and 3 cm long membrane tube. A reliable UPLC-MS/MS program was systematically optimized for the simultaneous detection of nine allelochemicals with different polarities. The results proved the differences in the contents and distributions of nine allelochemicals in three differentTaxussoils.