Sea urchin egg preparations as systems for the study of calcium-triggered exocytosis.
Sea urchin egg preparations as systems for the study of calcium-triggered exocytosis.
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海胆卵制剂作为钙触发胞吐作用研究的系统。
DOI：
10.1111/j.1469-7793.1999.00015.x
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Blank,PS
中科院分区：
文献类型：
--
作者：
Zimmerberg,J;Coorssen,JR;Vogel,SS;Blank,PS
本文综述了近年来本实验室在钙触发胞吐机制方面的研究进展。在棘皮动物受精卵过程中，皮质分泌囊胞大量同步分泌。通过将生理和分子分析与多种纯化的膜分离物相结合，这些分离物含有与质膜融合或相互融合的分泌囊泡，我们已经表征了这种钙触发的胞吐作用的最后步骤。我们的动力学分析导致了融合物的功能定义，其激活的钙遵循泊松统计。这种复合物的性质与存在于皮质囊泡系统中的异三聚体SNARE蛋白复合物的性质进行了比较。我们的数据不支持这种特殊的异三聚体复合体本身就是生物融合原的假设。
This paper reviews recent work in our laboratory on the mechanism of calcium‐triggered exocytosis. Upon echinoderm egg fertilization, cortical secretory vesicle exocytosis is massive and synchronous. By combining physiological and molecular analyses with a variety of purified membrane isolates containing secretory vesicles that fuse to the plasma membrane or each other, we have characterized the final steps of this calcium‐triggered exocytosis. Our kinetic analysis led to a functional definition of the fusion complex whose activation by calcium follows Poisson statistics. The properties of this complex are compared with the properties of the heterotrimeric SNARE protein complex that is present in the cortical vesicle system. Our data do not support the hypothesis that this particular heterotrimeric complex is by itself the biological fusogen.