Quercetin Suppresses Proliferation of Liver Cancer Cell Lines In Vitro
Quercetin Suppresses Proliferation of Liver Cancer Cell Lines In Vitro
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DOI：
10.21873/anticanres.14469
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发表时间：
2020-08-01
影响因子：
2
通讯作者：
Yano, Hirohisa
中科院分区：
文献类型：
--
作者：
Hisaka, Toru;Sakai, Hisamune;Yano, Hirohisa
背景/目的：我们研究槲皮素对肝癌细胞系的抗增殖作用。材料与方法：培养13个肝癌细胞系，然后用不同浓度的槲皮素（0-100 μ M）或槲皮素和5-FU处理，并通过MTT测定分析细胞活力。流式细胞术也被用来检查细胞周期进程后处理槲皮素。结果：槲皮素的加入导致细胞增殖的剂量和时间依赖性抑制。在一些细胞系中，槲皮素和5-FU处理会产生额外或协同作用。大多数细胞系在细胞周期的不同阶段显示细胞周期停滞。结论：槲皮素通过诱导肝癌细胞凋亡和细胞周期阻滞抑制肝癌细胞增殖。
Background/Aim: We investigated the anti-proliferative effect of quercetin on liver cancer cell lines. Materials and Methods: Thirteen liver cancer cell lines were cultured followed by treatment with varying concentrations of quercetin (0-100 mu M) or quercetin and 5-FU, and the cell viability was analysed by the MTT assay. Flow cytometry was also used to examine cell cycle progression after treatment with quercetin. Results: The addition of quercetin resulted in a dose- and time-dependent suppression of cell proliferation. In some cell lines, treatment with quercetin and 5-FU caused an additional or synergistic effect. Most cell lines displayed cell cycle arrest at different phases of the cell cycle. Conclusion: Quercetin inhibits the proliferation of liver cancer cells via induction of apoptosis and cell cycle arrest.