Intracellular Fluorescent Temperature Probe Based on Triarylboron Substituted Poly N-Isopropylacrylamide and Energy Transfer
Intracellular Fluorescent Temperature Probe Based on Triarylboron Substituted Poly N-Isopropylacrylamide and Energy Transfer
复制标题
基于三芳基硼取代聚N-异丙基丙烯酰胺的细胞内荧光温度探针及能量传递
DOI：
10.1021/acs.analchem.5b00887
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发表时间：
2015
影响因子：
7.4
通讯作者：
Yang Guoqiang
中科院分区：
文献类型：
--
作者：
Liu Jun;Guo Xudong;Hu Rui;Xu Jian;Wang Shuangqing;Li Shayu;Li Yi;Yang Guoqiang
设计并合成了一种基于极性敏感三芳基硼化合物（DPTB）和PNIPAM的新型亲水性荧光温度探针（PNDP）。为了克服基于单一强度的传感机制的缺点并获得更稳健的信号，通过在DPTB和尼罗红（NR）之间设计高效的FRET系统（PNDP-NR）来实现比率读出。PNDP-NR具有温度范围宽、线性关系好、温度分辨率高、可逆性好、稳定性好等优点。在30-55 °C的传感温度范围内，PNDP-NR的荧光颜色经历从红色到绿-蓝色的显著变化。PNDP-NR也被引入NIH/3 T3细胞中，以在单细胞水平上感知温度。它在体内具有优异的光稳定性和低细胞毒性。
A novel hydrophilic fluorescence temperature probe (PNDP) based on polarity-sensitive triarylboron compound (DPTB) and PNIPAM is designed and synthesized. In order to overcome the shortcomings of the single-intensity-based sensing mechanism and obtain more robust signals, ratiometric readout is achieved by designing an efficient FRET system (PNDP-NR) between DPTB and Nile Red (NR). PNDP-NR possesses some excellent features, including wide temperature range, good linear relationship, high temperature resolution, excellent reversibility, and stability. Within a sensing temperature range of 30–55 °C, the fluorescence color of PNDP-NR experiences significant change from red to green-blue. PNDP-NR is also introduced into NIH/3T3 cells to sense the temperature at the single-cell level. It gave excellent photostability and low cytotoxicity in vivo.