Morphoquantitative effects of acute diabetes on the myenteric neurons of the proximal colon of adult rats
Morphoquantitative effects of acute diabetes on the myenteric neurons of the proximal colon of adult rats
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DOI：
10.1590/s0004-282x2002000400012
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发表时间：
2002-09-01
影响因子：
1.4
通讯作者：
Miranda Neto, Marcílio H. de
中科院分区：
文献类型：
--
作者：
Furlan, Maria Montserrat D.P.;Molinari, Sônia L.;Miranda Neto, Marcílio H. de
研究了急性糖尿病对成年大鼠近端结肠肌间神经元密度和大小的影响。注射链脲佐菌素后进行7天的观察期，在此期间糖尿病动物表现出体重减轻、食物和水摄入过多、尿债大量和高血糖。来自近端结肠的整体用吉姆萨和NADH黄酶技术染色，并且这些技术的使用使得可以验证糖尿病大鼠结肠中的神经元密度和肌间神经元的细胞体尺寸的减少。这些观察结果根据糖尿病的病理生理学和实验方案进行了讨论。
The effects of acute diabetes on the density and size of the myenteric neurons of the proximal colon of adult rats were investigated. The injection of streptozotocin was followed by a period of observation of seven days, during which the diabetic animals showed weight loss, excessive food and water intake, large urinary debt and hyperglicemia. The whole-mounts from the proximal colon were stained with the techniques of Giemsa and of the NADH-diaphorase, and the employment of these techniques made it possible to verify a decrease on the neuronal density and on the cell body size of the myenteric neurons in the colon of the diabetic rats. These observations were discussed in terms of the pathophysiology of the diabetes and the experimental protocol.