Primed fibroblasts and exogenous decorin: Potential treatments for subacute vocal fold scar
Primed fibroblasts and exogenous decorin: Potential treatments for subacute vocal fold scar
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DOI：
10.1016/j.otohns.2006.07.011
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发表时间：
2006-12-01
影响因子：
3.4
通讯作者：
Hebda, Patricia A.
中科院分区：
文献类型：
--
作者：
Krishna, Priya;Rosen, Clark A.;Hebda, Patricia A.
目的：探讨肝细胞生长因子（HGF）引发的成纤维细胞和核心蛋白聚糖应用于皮肤和声带成纤维细胞在体外和体内在兔声带scar model.Study设计和设置：声带和皮肤成纤维细胞进行了五个在体外治疗条件：控制，表皮生长因子，HGF，核心蛋白聚糖和HGF，和核心蛋白聚糖单独。进行透明质酸和胶原酶联免疫吸附测定。12只家兔行单侧声带剥脱术。然后用皮肤成纤维细胞、HGF、HGF引发的成纤维细胞和核心蛋白聚糖或核心蛋白聚糖注射损伤的声带。结果：在体外培养条件下，HGF可促进声带成纤维细胞透明质酸的合成（P < 0.001）。HGF和核心蛋白聚糖治疗组胶原分泌减少（P < 0.01）。在体内，组织学结果表明治疗组之间的胶原含量差异很小。结论：HGF和核心蛋白聚糖一起可能会减少皮肤和声带成纤维细胞的胶原生成。成纤维细胞移植到有疤痕的声带中是否有益尚不明确。(c)2006年美国耳鼻咽喉头颈外科学会基金会。All rights reserved.
OBJECTIVE: To investigate hepatocyte growth factor (HGF) primed fibroblasts and decorin application on skin and vocal fold fibroblasts in vitro and in vivo in rabbit vocal fold scar model.STUDY DESIGN AND SETTING: Vocal fold and skin fibroblasts underwent five in vitro treatment conditions: control, epidermal growth factor, HGF, both decorin and HGF, and decorin alone. Hyalurome acid and collagen enzyme-linked immunosorbent assays were performed. In vivo, 12 rabbits underwent unilateral vocal fold stripping. Injured vocal folds were then injected with skin fibroblasts, HGF, HGF-primed fibroblasts and decorin, or decorin. Outcomes included histologic and lamina propria height analyses.RESULTS: in vitro, HGF increased hyaluronic acid synthesis in vocal fold fibroblasts (P < 0.001). HGF and decorin treatment diminished collagen secretion (P < 0.01). In vivo, histologic findings indicated minimal difference in collagen amount between treatment groups.CONCLUSION: HGF and decorin together may decrease collagen production by skin and vocal fold fibroblasts. Fibroblast transplantation into scarred vocal folds has equivocal benefit. (c) 2006 American Academy of Otolaryngology-Head and Neck Surgery Foundation. All rights reserved.