CHOLERA-TOXIN STIMULATES SECRETION OF IMMUNOREACTIVE INTESTINAL MUCIN
CHOLERA-TOXIN STIMULATES SECRETION OF IMMUNOREACTIVE INTESTINAL MUCIN
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DOI：
10.1152/ajpgi.1981.240.1.g10
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发表时间：
1981-01-01
影响因子：
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通讯作者：
FORSTNER, GG
中科院分区：
文献类型：
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作者：
FORSTNER, JF;ROOMI, NW;FORSTNER, GG
用粘蛋白双抗体放射免疫法测定大鼠小肠杯状细胞体外分泌粘蛋白的能力。将霍乱弧菌毒素（12.5-50 mg粗滤液/ml）加入肠切片孵育液中，引起粘蛋白分泌的剂量依赖性增加。到90分钟时，分泌量比未接受霍乱治疗的对照组增加了4至5倍。粗滤液（透析或非透析）是一种比纯化肠毒素更有效的粘蛋白促分泌剂。还通过在体内对大鼠腹膜内给予[1- 14 C]葡糖胺并在3小时后监测肠切片放射性糖蛋白的体外分泌来评估分泌。霍乱滤液（12.5-50毫克/毫升）引起的1.5- 2.0倍增强分泌90分钟后。放射性数据低估了总粘蛋白分泌和依赖性的分泌霍乱滤液的剂量。霍乱制剂还导致[3 H]葡萄糖胺掺入组织酸可沉淀糖蛋白的增强（比对照组增加20-30%），表明糖蛋白合成受到刺激。在相同的实验中，注意到3 H-标记的（即，新糖基化的）糖蛋白比未处理的对照增加2.5- 3.0倍。霍乱毒素可能既促进储存颗粒中旧粘蛋白的释放，又促进培养过程中杯状细胞中新粘蛋白的合成和分泌。
In vitro secretion of goblet cell mucin from rat small intestine was measured using a double-antibody radioimmunoassay for mucin. Cholera (Vibria cholerae) toxin (12.5-50 mg crude filtrate/ml) added to incubations of intestinal slices caused a dose-dependent increase in mucin secretion. By 90 min there was a 4- to 5-fold enhancement in secretion over noncholera-treated controls. Crude filtrate (dialyzed or nondialyzed) was a more effective mucin secretogogue than purified enterotoxin. Secretion was also assessed by administering [1-14C]glucosamine i.p. to rats in vivo and 3 h later monitoring in vitro secretion of radioactive glycoprotein from intestinal slices. Cholera filtrate (12.5-50 mg/ml) caused a 1.5- to 2.0-fold enhancement in secretion after 90 min. The radioactivity data underestimated total mucin secretion and the dependency of secretion on the dose of cholera filtrate. Cholera preparations also caused an enhancement (20-30% over controls) in the incorporation of [3H]glucosamine into tissue acid-precipitable glycoprotein, indicating a stimulation of glycoprotein synthesis. In the same experiments it was noted that the secretion of 3H-labeled (i.e., newly glycosylated) glycoprotein was increased 2.5- to 3.0-fold over untreated controls. It is possible that cholera toxin promotes the release of both old mucin from storage granules and the synthesis and secretion of new mucin formed in goblet cells during incubation.