Controlling microarray spot morphology with polymer liftoff arrays.
Controlling microarray spot morphology with polymer liftoff arrays.
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DOI：
10.1021/ac061898z
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发表时间：
2007-02
影响因子：
7.4
通讯作者：
J. Moran-Mirabal;Christine P. Tan;R. Orth;Eric O. Williams;H. Craighead;D. M. Lin
中科院分区：
文献类型：
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作者：
J. Moran-Mirabal;Christine P. Tan;R. Orth;Eric O. Williams;H. Craighead;D. M. Lin
生物阵列受到生物材料沉积不均匀的阻碍。旨在改善这种沉积的努力集中在改变溶液的组成或用于存款材料的工具上。然而，很少有人注意到通过限制表面的物理和化学形貌来控制材料沉积。在这里，我们提出了使用一个混合的亲水/疏水微图案化的表面，以指导沉积点DNA的微阵列。这些聚合物“剥离”阵列将二对二甲苯（Parylene）的疏水性表面特性与聚合物内的光刻蚀刻亲水性开口结合起来，联合收割机。我们表明，这些基板上的溶质的流动模式有利于浓度的溶解材料到介观开口下的印刷点，从而显着提高沉积的均匀性。此外，微图案化的表面允许增加斑点材料的复制。最后，这些聚合物剥离阵列显示出减少的阵列间变化，提高了数据采集的再现性。我们设想，这些新的基板可以推广到其他图案化的生物材料产生更均匀的阵列。
Biological arrays are hindered by the lack of uniformity in the deposition of biomaterials. Efforts aimed at improving this deposition have focused on altering the composition of the solution or the tool used to deposit the material. However, little attention has been paid to controlling material deposition by constraining the physical and chemical topography of the surface. Here we present the use of a hybrid hydrophilic/hydrophobic micropatterned surface to direct the deposition of spotted DNA on microarrays. These polymer "liftoff" arrays combine the hydrophobic surface properties of di-p-xylylene (Parylene) with photolithographically etched hydrophilic openings within the polymer. We show that the flow pattern of solutes on these substrates favors the concentration of dissolved material into the mesoscopic openings underlying the printed spot, resulting in significantly improved uniformity of deposition. Moreover, the micropatterned surface allows for increased replication of spotted materials. Finally, these polymer liftoff arrays display reduced array-to-array variation, improving the reproducibility of data acquisition. We envision that these novel substrates can be generalized to produce more uniform arrays of other patterned biomaterials.