BIOGENESIS OF ESCHERICHIA-COLI PAP PILI - PAPH, A MINOR PILIN SUBUNIT INVOLVED IN CELL ANCHORING AND LENGTH MODULATION
BIOGENESIS OF ESCHERICHIA-COLI PAP PILI - PAPH, A MINOR PILIN SUBUNIT INVOLVED IN CELL ANCHORING AND LENGTH MODULATION
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DOI：
10.1016/0092-8674(87)90565-4
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发表时间：
1987-04-24
期刊：
影响因子：
64.5
通讯作者：
NORMARK, S
中科院分区：
文献类型：
--
作者：
BAGA, M;NORGREN, M;NORMARK, S
研究了由 pap 基因簇编码的大肠杆菌 Pap pili 的生物发生。鉴定出一种新基因 papH，并发现其编码弱表达的菌毛蛋白样蛋白。 PapH对于双半乳糖苷特异性结合和巴氏菌毛的形成是可有可无的。然而，在 papH 缺失突变体中，发现总菌毛抗原的 50%-70% 不含细胞。我们提出的证据表明 papH 和相邻基因 papA 存在共调节，papA 编码主要菌毛蛋白亚基。 PapA 与 PapH 比率的降低导致大部分细胞产生菌毛缩短，而 PapA 相对于 PapH 的过量产生导致细胞菌毛延长。数据显示 PapH 在将菌毛锚定到细胞和调节菌毛长度方面发挥作用。
The biogenesis of Escherichia coli Pap pili, encoded by the pap gene cluster, was studied. A novel gene, papH, was identified and found to encode a weakly expressed pilin-like protein. PapH was dispensable for digalactoside-specific binding and for formation of Pap pili. However, in papH deletion mutants 50%-70% of total pilus antigen was found free of the cells. We present evidence showing coregulation of papH and the adjacent gene, papA, which encodes the major pilin subunit. A decrease in the PapA to PapH ratio resulted in a large fraction of cells producing shortened pili, whereas overproduction of PapA relative to PapH resulted in cells with lengthened pili. The data show that PapH has roles in anchoring the pilus to the cell and in modulating pilus length.