Corticotropin-releasing hormone differentially modulates the interleukin-1 system according to the level of monocyte activation by endotoxin.
Corticotropin-releasing hormone differentially modulates the interleukin-1 system according to the level of monocyte activation by endotoxin.
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促肾上腺皮质激素释放激素根据内毒素对单核细胞的激活水平差异性地调节白介素-1 系统。
DOI：
10.1210/endo.136.12.7588301
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发表时间：
1995
期刊：
影响因子：
4.8
通讯作者：
E. Arzt
中科院分区：
文献类型：
--
作者：
M. Pereda;J. Sauer;C. Castro;S. Finkielman;G. Stalla;F. Holsboer;E. Arzt
白细胞介素-1（IL-1）系统由IL-1 α和IL-1 β以及结合相同IL-1受体的IL-1受体拮抗剂（IL-1 ra）构成。下丘脑-垂体-肾上腺轴激素是神经内分泌调节免疫功能的主要介质。促肾上腺皮质激素释放激素（CRH）产生于外周炎症部位，但其对炎症细胞因子合成的直接影响尚不清楚。我们研究了CRH（0.1-100 nM）对不同剂量脂多糖（LPS）激活培养的人外周血单核细胞IL-1 β和IL-1 ra表达的影响。在没有LPS的情况下，CRH上调IL-1 ra和IL-1 β信使RNA表达以及蛋白质合成。低剂量LPS（1 ng/ml）未观察到显著变化。相反，与高剂量的LPS（1微克/毫升）组合，CRH引起IL-1 ra和IL-1 β转录和分泌的抑制。CRH的作用可被其拮抗剂α-螺旋CRH阻断，并由胞内cAMP介导。这些数据表明，CRH调节IL-1系统，根据单核细胞的激活状态，CRH对激活的细胞施加抑制控制，对静息单核细胞施加刺激作用。
The interleukin-1 (IL-1) system is constituted by IL-1 alpha and IL-1 beta and IL-1 receptor antagonist (IL-1ra) that bind the same IL-1 receptors. Hypothalamic-pituitary-adrenal axis hormones are major mediators of the neuroendocrine control over immune function. Corticotropin-releasing hormone (CRH) is produced in peripheral inflammatory sites; its direct effects on inflammatory cytokine synthesis, however, remain unclear. We have studied the effects of CRH (0.1-100 nM) on IL-1 beta and IL-1ra expression by human peripheral monocytes in culture activated with different doses of lipopolysaccharide (LPS). In the absence of LPS, CRH up-regulated IL-1ra and IL-1 beta messenger RNA expression as well as protein synthesis. No significant changes were observed with low doses of LPS (1 ng/ml). In contrast, in combination with high doses of LPS (1 microgram/ml), CRH caused inhibition of IL-1ra and IL-1 beta transcription and secretion. The CRH effects were blocked by its antagonist alpha-helical CRH and mediated by intracellular cAMP. These data indicate that CRH modulates the IL-1 system; depending on the state of activation of the monocyte, CRH exerts an inhibitory control on the activated cell and a stimulatory action on the resting monocyte.
影响因子：
20.3
作者：
E. Fischer;K. J. V. Zee;Michael A. Marano;C. S. Rock;John S. Kenney;D. Poutsiaka;C. A. Dinarello;Stephen F. Lowry;L. Moldawer
通讯作者：
E. Fischer;K. J. V. Zee;Michael A. Marano;C. S. Rock;John S. Kenney;D. Poutsiaka;C. A. Dinarello;Stephen F. Lowry;L. Moldawer
IL-4 相互调节人单核细胞中 IL-1 和 IL-1 受体拮抗剂的表达。
DOI：
--
发表时间：
1992
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Fenton,MJ;Buras,JA;Donnelly,RP
通讯作者：
Donnelly,RP