CHARACTERIZATION OF THE GENES OF THE 2,3-BUTANEDIOL OPERONS FROM KLEBSIELLA-TERRIGENA AND ENTEROBACTER-AEROGENES
CHARACTERIZATION OF THE GENES OF THE 2,3-BUTANEDIOL OPERONS FROM KLEBSIELLA-TERRIGENA AND ENTEROBACTER-AEROGENES
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DOI：
10.1128/jb.175.5.1392-1404.1993
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发表时间：
1993-03-01
影响因子：
3.2
通讯作者：
PENTTILA, ME
中科院分区：
文献类型：
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作者：
BLOMQVIST, K;NIKKOLA, M;PENTTILA, ME
从土克雷伯菌中分离出参与 2,3-丁二醇途径编码 α-乙酰乳酸脱羧酶、α-乙酰乳酸合酶 (α-ALS) 和乙偶姻（二乙酰）还原酶的基因，并显示位于一个操纵子中。该操纵子也被证明存在于产气肠杆菌中。 budA 基因编码 α-乙酰乳酸脱羧酶，在两种生物体中产生 259 个氨基酸的蛋白质。这些蛋白质之间的氨基酸相似性为87%。对分别编码α-ALS和乙偶姻还原酶的K. terrigena基因budB和budC进行了测序。 559 个氨基酸长的 α-ALS 酶与由基因 ilvB、ilvG 和 ilvI 编码的大肠杆菌合成代谢 α-ALS 酶的大亚基相似。 K. terrigena α-ALS 还被证明可以补充合成代谢 α-ALS 缺陷的大肠杆菌菌株来合成缬氨酸。 243个氨基酸长的乙偶姻还原酶具有昆虫型乙醇脱氢酶/核糖醇脱氢酶家族的共有氨基酸序列，并且与三种已知脱氢酶和一种氧化还原酶的N末端和内部区域具有广泛的相似性。
The genes involved in the 2,3-butanediol pathway coding for alpha-acetolactate decarboxylase, alpha-acetolactate synthase (alpha-ALS), and acetoin (diacetyl) reductase were isolated from Klebsiella terrigena and shown to be located in one operon. This operon was also shown to exist in Enterobacter aerogenes. The budA gene, coding for alpha-acetolactate decarboxylase, gives in both organisms a protein of 259 amino acids. The amino acid similarity between these proteins is 87%. The K. terrigena genes budB and budC, coding for alpha-ALS and acetoin reductase, respectively, were sequenced. The 559-amino-acid-long alpha-ALS enzyme shows similarities to the large subunits of the Escherichia coli anabolic alpha-ALS enzymes encoded by the genes ilvB, ilvG, and ilvI. The K. terrigena alpha-ALS is also shown to complement an anabolic alpha-ALS-deficient E. coli strain for valine synthesis. The 243-amino-acid-long acetoin reductase has the consensus amino acid sequence for the insect-type alcohol dehydrogenase/ribitol dehydrogenase family and has extensive similarities with the N-terminal and internal regions of three known dehydrogenases and one oxidoreductase.