A family of antimicrobial and immunomodulatory peptides related to the frenatins from skin secretions of the Orinoco lime frog Sphaenorhynchus lacteus (Hylidae)
A family of antimicrobial and immunomodulatory peptides related to the frenatins from skin secretions of the Orinoco lime frog Sphaenorhynchus lacteus (Hylidae)
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DOI：
10.1016/j.peptides.2014.03.020
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发表时间：
2014-06-01
期刊：
影响因子：
3
通讯作者：
McClean, Stephen
中科院分区：
文献类型：
--
作者：
Conlon, J. Michael;Mechkarska, Milena;McClean, Stephen
对去甲肾上腺素刺激的青蛙Sphanowhynchus lacteus(Hylidae，Hylinae)皮肤分泌物的多肽分析表明，存在三个与Litoria infrenata(Hylidae，Pelodryadinae)的Frenatin 2和Discoglossus Sardus(Alytidae)的Frenatin 2D具有有限序列相似性的结构相关的宿主防御肽。Frenatin 2.1S(GLVGTLLGHIGKAILG中心点NH2)和Frenatin 2.2S(GLVGTLLGHIGKAILS中心点NH2)在C端是氨基化的，而Frenatin 2.3S(GLVGTLGHIGKAILG)不是。Frenatin 2.1s和2.2s对临床分离的耐甲氧西林金黄色葡萄球菌(MRSA)和表皮葡萄球菌(MIC)具有较强的杀菌活性
Peptidomic analysis of norepinephrine-stimulated skin secretions of the Orinoco lime tree frog Sphaenorhynchus lacteus (Hylidae, Hylinae) revealed the presence of three structurally related host-defense peptides with limited sequence similarity to frenatin 2 from Litoria infrafrenata (Hylidae, Pelodryadinae) and frenatin 2D from Discoglossus sardus (Alytidae). Frenatin 2.1S (GLVGTLLGHIGKAILG center dot NH2) and frenatin 2.2S ( GLVGTLLGHIGKAILS center dot NH2) are C-terminally a-amidated but frenatin 2.3S ( GLVGTLLGHIGKAILG) is not. Frenatin 2.1S and 2.2S show potent bactericidal activity against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus epidermidis (MIC