LOCALIZATION OF TUBULIN IN MITOTIC APPARATUS OF MAMMALIAN-CELLS BY IMMUNOFLUORESCENCE AND IMMUNOELECTRON MICROSCOPY
LOCALIZATION OF TUBULIN IN MITOTIC APPARATUS OF MAMMALIAN-CELLS BY IMMUNOFLUORESCENCE AND IMMUNOELECTRON MICROSCOPY
复制标题
DOI：
10.1007/bf00329772
复制
发表时间：
1977-01-01
期刊：
影响因子：
1.6
通讯作者：
BRINKLEY, BR
中科院分区：
文献类型：
--
作者：
PEPPER, DA;BRINKLEY, BR
用抗微管蛋白抗体作为免疫荧光和免疫电子显微镜探针，在体外对大鼠袋鼠(品系PtK1)细胞有丝分裂器中的微管蛋白进行了定位。除了在纺锤体微管和中心粒中检测到微管蛋白外，其他结构还显示出特殊的染色，包括动心、无定形的着丝粒周围物质和与中心粒相关的小病毒样颗粒。动色素由一层约400埃厚的染色浓密的外层组成，该外层与相同尺寸的内层之间隔着一层浅染的中间层。微管主要分布于动粒最外板，但微管蛋白均匀分布于外板和内板。Colcemid处理阻止了纺锤体微管的组装，并导致了动粒的特异性改变，但未能减少动粒的染色。微管蛋白分子可能构成着动粒的重要结构成分。
Antitubulin antibody was used as an immunofluorescent and immunoelectron microscopic probe to localize tubulin in components of the mitotic apparatus of rat kangaroo (strain PtK1) cells in vitro. In addition to the detection of tubulin in the spindle microtubules and centrioles, other structures displayed specific staining including kinetochores, amorphous pericentriolar material and small virus-like particles associated with the centrioles. The kinetochroes consisted of a densely stained outer layer about 400 .ANG. thick which is separated from an inner layer of the same dimension by a lightly staining middle layer. Microtubules were primarily associated with the outermost plate of the kinetochore but tubulin was uniformly distributed in both outer and inner plates. Colcemid treatment prevented the assembly of spindle microtubules and resulted in specific alterations of the kinetochore but failed to diminish the staining of the kinetochores. Tubulin molecules may comprise an important structural component of the kinetochore.