Effect of cytosol fractions from lutropin-stimulated rat testes on pregnenolone production by mitochondria from normal rat testes.
Effect of cytosol fractions from lutropin-stimulated rat testes on pregnenolone production by mitochondria from normal rat testes.
复制标题
促黄体素刺激的大鼠睾丸细胞质组分对正常大鼠睾丸线粒体产生孕烯醇酮的影响。
DOI：
10.1016/0304-4165(78)90068-5
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
H. van der Molen
中科院分区：
文献类型：
--
作者：
C. Bakker;M. P. van der Plank;H. van der Molen
睾丸中产生类固醇的速率限制步骤是线粒体将胆固醇转化为孕烯醇酮。这种转化可以由lutropin刺激，但是lutropin诱导的细胞质因子和线粒体活性在类固醇生产中的确切相互作用尚不清楚。本论文所描述的结果是关于用从总睾丸匀浆中分离的上清组分重组实验中分离的线粒体组分的类固醇生产。氰酮以及类固醇17α-羟化酶活性抑制剂SU-10603是阻断孕烯醇酮代谢所必需的。结果表明，细胞质中含有lutropin-induced factor(s)，该因子可在体外对对照睾丸完整线粒体胆固醇侧链裂解活性产生影响。
The rate limiting step in the production of steroids in the testis is the mitochondrial conversion of cholesterol to pregnenolone. This conversion can be stimulated by lutropin, but the precise interaction between lutropin-induced cytoplasmic factors and the mitochondrial activity in steroid production is as yet unknown. The results described in the present paper concern the steroid production of isolated mitochondrial fractions in recombination experiments with isolated supernatant fractions from total testes homogenates. Cyanoketone as well as SU-10603, an inhibitor of steroid 17α-hydroxylase activity are required to block pregnenolone metabolism. The results show that the cytoplasm contains lutropin-induced factor(s) which can exert its effect in vitro on the cholestorel side-chain cleavage activity in intact mitochondria isolated from control testes.