STAGE-SPECIFIC PATTERNS OF COLLAGEN GENE-EXPRESSION DURING DEVELOPMENT OF CAENORHABDITIS-ELEGANS
STAGE-SPECIFIC PATTERNS OF COLLAGEN GENE-EXPRESSION DURING DEVELOPMENT OF CAENORHABDITIS-ELEGANS
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DOI：
10.1128/mcb.5.2.363
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发表时间：
1985-01-01
影响因子：
5.3
通讯作者：
HIRSH, D
中科院分区：
文献类型：
--
作者：
COX, GN;HIRSH, D
胶原蛋白是C.线虫的表皮是由40-150个密切相关但不相同的基因组成的大家族编码的。mRNA积累的时间模式，确定了大量的胶原蛋白基因的筛选重组体和质粒含有克隆的胶原蛋白基因在高严格的条件下与32 P-标记的C[互补]DNA制剂具体的鸡蛋或3胚后蜕皮。在C. elegans发育大多数基因的研究表现出1 4模式的mRNA积累与角质层形态和胶原蛋白组成的变化在发展过程中。一般来说，在正常发育过程中，新的胶原蛋白基因似乎会逐渐激活。
Collagens are the major protein components of the C. elegans cuticle and are encoded by a large family of 40-150 closely related but nonidentical genes. Temporal patterns of mRNA accumulation were determined for a large number of collagen genes by screening recombinant phages and plasmids containing cloned collagen genes under high stringency conditions with 32P-labeled c[complementary]DNA preparations specific for eggs or 3 postembryonic molts. Collagen mRNA levels are regulated both temporally and quantitatively during C. elegans development. Most genes studied exhibit 1 of 4 patterns of mRNA accumulation which correlate with changes in cuticle morphology and collagen protein composition during development. In general, it seems there is a progressive activation of new collagen genes during normal development.