Transplantation of menstrual blood-derived mesenchymal stem cells (MbMSCs) promotes the regeneration of mechanical injuried endometrium
Transplantation of menstrual blood-derived mesenchymal stem cells (MbMSCs) promotes the regeneration of mechanical injuried endometrium
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经血间充质干细胞（MbMSCs）移植促进机械损伤子宫内膜再生
DOI：
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
long Sui
中科院分区：
文献类型：
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作者：
Limei Chen;Luopei Guo;Fang Chen;Yu Xie;hongwei Zhang;peiqing Quan;long Sui
目的：宫腔粘连的根本原因是子宫内膜的实质性破坏，使子宫内膜再生困难。本研究旨在观察经血来源的间充质干细胞（MbMSCs）不同移植方法对子宫内膜再生的影响。我们还研究了转染FGF2基因的MbMSCs是否可以提高再生效果。方法：采用SD雌性大鼠75只，切除子宫内膜，建立IUA模型。将IUA模型分为5组：A组为IUA对照组，B组为支架移植组，C组为支架+MbMSC移植组，D组为支架+FGF 2-MbMSC移植组，E组为经尾静脉注射FGF 2-MbMSC组。干预后第7天和第28天每组各处死5只大鼠。用增强型绿色荧光蛋白示踪MbMSCs在子宫内膜中的分布。观察子宫内膜形态、内膜腺体数量、内膜纤维化面积及Ki67、VEGF、CD31免疫组化（IHC）。此外，对所有组的生育力进行了测试。结果如下：移植后第7天，增强型绿色荧光蛋白检测显示，D组子宫腔中MbMSCs数量明显多于E组。A组和B组子宫内膜形态萎缩变薄，纤维化比例高。C、D和E组子宫内膜增厚，腺体较多，纤维化程度减轻，Ki67、VEGF和CD31表达增加。子宫内膜再生效果由高到低为D> C> E，两组间差异有统计学意义。。生育力测试验证了再生效果。结论：结果提示，MbMSCs经尾静脉注射是一种有效的促进子宫内膜再生的方法，但其效果不如MbMSCs与支架材料联合宫内移植。转染FGF2基因的骨髓间充质干细胞具有更强的再生能力。
Purpose: The ultimate cause of intrauterine adhesions (IUAs) is the substantial destruction of the endometrium, which makes the regeneration of endometrium difficult. The purpose of this study was to observe menstrual blood-derived mesenchymal stem cells (MbMSCs)’s effect on the endometrial regeneration with different methods of transplantation. We also studied whether MbMSCs transfected with the FGF2 gene can improve the regenerative effect. Methods: 75 female SD rats with endometrium removed were used as IUA models. These IUA models were divided into 5 groups: group A was the IUA control, group B received a scaffold transplant, group C received a scaffold+MbMSC transplant, group D received a scaffold+FGF2-MbMSC transplant, and group E received FGF2-MbMSCs injected via the tail vein. After the intervention, 5 rats from each group were sacrificed on the 7th day and .the 28th day respectively. The distribution of MbMSCs in endometrium was traced using enhanced green fluorescent protein. The endometrial morphology, number of endometrial glands, area of endometrial fibrosis and immunohistochemistry (IHC) of Ki67, VEGF, and CD31 were evaluated. In addition, the fertility of all groups was tested. Results: On the 7th day after transplantation, enhanced green fluorescent protein showed that there were more MbMSCs in .the uterine cavity of group D than that of group E. The endometrial morphology of groups A and B was atrophic and thinned with a high proportion of fibrosis in the endometrium. The endometrium of groups C, D and E was thickened, contained more glands, exhibited reduced fibrosis, and had increased expression of Ki67, VEGF and CD31. The endometrial regenerative effect from high to low was D > C > E with significant differences between each two groups. .The fertility test verified the regenerative effect. Conclusions: These results suggest that the injection of MbMSCs into the tail vein was an effective way to stimulate endometrial regeneration, but the effect was not so well as the intrauterine transplant of MbMSCs with scaffold. The FGF2-transfected MbMSCs exhibited enhanced regenerative effect.