Rapid Micropropagation of Sapium Sebiferum using Segments with Axillary Bud for Forest Bioenergy
Rapid Micropropagation of Sapium Sebiferum using Segments with Axillary Bud for Forest Bioenergy
复制标题
DOI：
--
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Yawei Xie
中科院分区：
文献类型：
--
作者：
Youming Xu;Ningning Wang;Yawei Xie
Sapium sebiferum is one of the four woody oil tree species in China for bioenergy forest. Its seeds have high oil content and are widely used. It is an excellent tree species that can be cultivated as a renewable energy source. An experiment was conducted to study culture in vitro and rapid propagation of S. sebiferum using segments with axillary bud. The results showed that the survival rate could be reached to 50.0% when the explants were disinfected with 70% ethanol for 30 seconds and with 0.1% HgCl2 for 10 minutes. In induction culture, the lateral buds began to sprout about 5 days later, .and after 20 days, the buds gradually differentiated. Among the three basic mediums, WPM medium has the highest effective bud induction rate, the effect of 6-benzyladenine (6-BA) mass concentration on axillary bud induction rate is extremely significant. The optimal medium WPM +6-BA 0.5mg/L+ NAA 0.05mg/L, conducting bud induction, and the induction rate could reach 62.9% after 30 days. Multiplication culture was carried out in the medium MS + 6-BA 1.0 mg/L +NAA 0.5 mg/L+ KT 0.5 mg/L, the proliferation effect was the best, with the multiplication rate of 81.2% and proliferation multiple of 3.2. The results of variance analysis showed that Naphthalene acetic acid (NAA) is the main factor affecting bud .proliferation, followed by 6-BA. The rooting rate was up to 93.3% after induction in the medium 1/2 MS+ IBA 0.5mg/L. The establishment of rapid propagation technology system through tissue culture is an important way to solve the shortage of high-quality seedlings and provide technical support for the establishment of large scale S. sebiferum energy forest.
