The effects of retinoic acid on ciliary function of regenerated sinus mucosa
The effects of retinoic acid on ciliary function of regenerated sinus mucosa
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DOI：
10.2500/ajr.2008.22.3176
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发表时间：
2008-05-01
期刊：
影响因子：
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通讯作者：
Hwang, Peter H.
中科院分区：
文献类型：
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作者：
Erickson, Vanessa R.;Antunes, Marcelo;Hwang, Peter H.
背景：与对照组相比，维甲酸(RA)可增强再生鼻窦粘膜的纤毛超微结构。然而，再生纤毛的功能状态还没有得到研究。目的：观察外用维甲酸对兔上颌窦再生黏膜纤毛搏动频率(CBF)的影响。方法：12只兔行双侧上颌窦剥离手术，右侧用维甲酸凝胶处理，左侧用惰性凝胶对照。于术后2周或4周处死动物，对再生黏膜进行CBF分析。3只未手术的兔建立标准的CBF数据。结果：11/12个RA处理的鼻窦和12/12个凝胶对照的鼻窦恢复了功能性纤毛。术后2周，RA处理组的平均CBF为19.78赫兹，与正常对照组比较差异有统计学意义(P<；0.26)。惰性凝胶处理组的CBF为29.24赫兹，显著高于正常对照组(p<；0.05)。4周后，纤毛活动持续，但维甲酸处理的鼻窦和凝胶对照组的CBF均高于正常对照组(p<；0.03)。结论：局部维甲酸放置在去粘膜的上颌窦可产生功能性纤毛。与对照组相比，RA似乎在粘膜伤口愈合过程的早期对CBF有正常化作用。这种影响在伤口愈合的后期似乎有所缓解。RA在增强再生纤毛的形态和功能方面可能是有益的。
Background: Retinoic acid (RA) has been shown to enhance ciliary ultrastructure in regenerated sinus mucosa compared with controls. However, the functional status of the regenerated cilia has not been studied. Our objective was to evaluate the ciliary beat frequency (CBF) of regenerated sinus mucosa treated with topical RA in a rabbit model.Methods: Twelve rabbits underwent bilateral surgical stripping of the maxillary sinuses, followed by treatment with RA gel in the right side and an inert gel control in the left side. The rabbits were then killed at either 2 or 4 weeks, and CBF analysis of the regenerated mucosa was performed. Three unoperated rabbits were used to establish normative CBF data.Results: Functional cilia were recovered from 11/12 RA-treated sinuses and 12/12 gel control sinuses. At 2 weeks postoperatively, the RA-treated sinuses showed an average CBF of 19.78 Hz, which was statistically comparable with the normal unoperated controls (p < 0.26). The inert gel-treated mucosa showed a CBF of 29.24 Hz, which was significantly elevated compared with normals (p < 0.05). At 4 weeks, ciliary activity persisted, but both RA-treated sinuses and gel controls showed elevated CBF compared with normals (p < 0.03).Conclusion: Topical RA placed in a demucosalized maxillary sinus yields functional cilia. RA appears to have a normalizing effect on CBF early in the mucosal wound healing process compared with control. This effect appears to be mitigated in later stages of wound healing. RA may be beneficial in enhancing morphological and functional aspects of regenerating cilia.