STRUCTURAL VARIATIONS IN THE DROSOPHILA RETROTRANSPOSON, 17.6
STRUCTURAL VARIATIONS IN THE DROSOPHILA RETROTRANSPOSON, 17.6
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DOI：
10.1093/nar/14.12.4765
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发表时间：
1986-06-25
影响因子：
14.9
通讯作者：
SAIGO, K
中科院分区：
文献类型：
--
作者：
INOUYE, S;HATTORI, K;SAIGO, K
从果蝇反转录转座子17.6中分离到21个以上的成员，通过限制性内切酶图谱、斑点杂交、异源双链分析和长末端重复序列(LTRS)的核苷酸序列测定，研究了它们可能的结构变化。至少有7名成员被发现患有末端或内部长时间缺失。在一个元素内或元素之间都没有发现具有相同核苷酸序列的LTRs对。尽管在左侧的LTR中发现了可能的基因组大小的转录本17.6的起始点，这是反式转录的潜在底物，但我们的结果总体上支持这样的观点，即大多数17.6转录本已经在其目前的染色体座位上持续居住了很长一段时间，因此17.6的易位率非常低。
More than 21 members of 17.6, a Drosophila retrotransposon, were isolated and their possible structural changes were examined by restriction mapping, blot hybridization, heteroduplex analysis and nucleotide sequence determination of long terminal repeats (LTRs). At least 7 members were found to suffer with terminal or internal long deletions. No pair of LTRs having an identical nucleotide sequence was found either within an element or between elements. Although an initiation site for the presumable genome-sized transcript of 17.6, a potential substrate for reverse transcription on trans location, was identified within the left-hand LTR, our results as a whole support the notion that the majority of 17.6s have continued to reside for a long period of time at their present chromosomal loci and hence the rate of translocation of 17.6 is very low.