Enclosed chambers for humidity control and sample containment in fiber diffraction
Enclosed chambers for humidity control and sample containment in fiber diffraction
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DOI：
10.1107/s0021889807060803
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发表时间：
2008-02-01
影响因子：
6.1
通讯作者：
Stubbs, Gerald
中科院分区：
文献类型：
--
作者：
McDonald, Michele;Kendall, Amy;Stubbs, Gerald
描述了一种用于制造衍射纤维的室和拉伸架。该室由一个简单的塑料试管和氮化硅窗制成。它适用于在纤维干燥和数据收集过程中保持恒定的湿度，并允许在样品制备过程中拉伸纤维和暴露在磁场中。必要时，它为有毒和传染性生物材料提供初级防护。Ne室已用于丝状植物病毒和酵母朊蛋白的纤维衍射实验，并显示出在分子水平上产生优异的定向和维持水合作用和秩序。
A chamber and stretch frame for making fibers for diffraction is described. The chamber is made from a simple plastic cuvette with silicon nitride windows. It is suitable for maintaining constant humidity during fiber drying and data collection, and allows stretching of the fiber and exposure to magnetic fields during sample preparation. If necessary, it provides primary containment for toxic and infectious biological materials. ne chamber has been used in fiber diffraction experiments with filamentous plant viruses and a yeast prion protein, and is shown to produce excellent orientation and to maintain hydration and order at the molecular level.