Cyclic somatic embryogenesis and efficient plant regeneration from callus of safflower
Cyclic somatic embryogenesis and efficient plant regeneration from callus of safflower
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DOI：
10.1007/s10535-008-0087-3
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发表时间：
2008-09-01
影响因子：
1.5
通讯作者：
Castano, E.
中科院分区：
文献类型：
--
作者：
Kumar, J. Vijaya;Kumari, B. D. Ranjitha;Castano, E.
以红花（Carthamus tinctorius L.）CV. NARI-6。胚性愈伤组织的诱导从10至17天的子叶和叶片外植体从离体幼苗。子叶外植体在附加噻苯隆、2-异戊烯腺嘌呤和吲哚-3-丁酸的MS培养基上获得了高频率（94.3%）的胚性愈伤组织。胚性愈伤组织在不含植物生长调节剂的不同培养基中形成初生、次生和循环体细胞胚，然而，在MSG上培养的子叶衍生的胚性愈伤组织获得100%循环体细胞胚发生。体细胞胚在添加赤霉素的四分之一MSG培养基中成熟和萌发。带根极或无根极的子叶在生根培养基中转化为正常植株并产生不定根。使生根的植物适应环境并成功地转移到田间。
Efficient plant regeneration through somatic embryogenesis was established for safflower (Carthamus tinctorius L.) cv. NARI-6. Embryogenic calli were induced from 10 to 17-d-old cotyledon and leaf explants from in vitro seedlings. High frequency (94.3 %) embryogenic callus was obtained from cotyledon explants cultured on Murashige and Skoog's germination (MSG) basal medium supplemented with thidiazuron, 2-isopentenyladenine and indole-3-butyric acid. Primary, secondary and cyclic somatic embryos were formed from embryogenic calli in a different media free of plant growth regulators, however, 100 % cyclic somatic embryogenesis was obtained from cotyledon derived embryogenic calli cultured on MSG. Somatic embryos matured and germinated in quarter-strength MSG medium supplemented with gibberellic acid. Cotyledons with root poles or non root poles were converted to normal plantlets and produced adventitious roots in rooting medium. Rooted plants were acclimatized and successfully transferred to the field.