Chinese herbal ingredients are effective immune stimulators for chickens infected with the Newcastle disease virus
Chinese herbal ingredients are effective immune stimulators for chickens infected with the Newcastle disease virus
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DOI：
10.1093/ps/85.12.2169
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发表时间：
2006-12-01
期刊：
影响因子：
4.4
通讯作者：
Yang, C. -B.
中科院分区：
文献类型：
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作者：
Kong, X. -F.;Hu, Y. -L.;Yang, C. -B.
本研究通过体外和体内试验，确定了4种中药成分（CHI）作为活疫苗免疫刺激剂对鸡的免疫效果。CHI采用黄芪多糖（APS）、板蓝根多糖（IRPS）、蜂胶多糖和淫羊藿黄酮等不同浓度。选取14日龄雄性白罗马鸡200只，随机分为10组。1 ~ 9组鸡采用鼻内和眼内两种方式接种新城疫病毒（NDV） IV株疫苗。1 ~ 8组在颈部背侧皮下注射相应CHI 0.5 mL，剂量分别为：APS组和IRPS组分别为29和58 mg/kg体重，其他组分别为7.25和14.5 mg/kg体重，每日1次，连续3 d。第9组（无chi对照）和第10组（无chi对照和疫苗对照）分别注射生理盐水0.5 mL。采用已建立的方法定量测定了免疫鸡新堡病病毒特异性血清血凝抑制抗体（Ab）的产生。结果表明，在适当浓度下，大多数CHI能有效促进鸡胚成纤维细胞对新城病毒感染的体外增殖。与单独注射NDV相比，在体内给疫苗鸡注射CHI （7.25 ~ 58 mg/kg体重，取决于类型）可提高血清抗NDV血凝抑制抗体滴度浓度。对于所有CHI，在开始接种后的第21天观察到对Ab产生有益的影响。在体内剂量上，蜂胶多糖和淫羊藿黄酮对雏鸟体液免疫反应的促进作用强于黄芪多糖和IRPS （P < 0.05）。综上所述，这些发现表明，适当剂量的CHI可以作为鸡的新型、有效的免疫刺激剂。
This study was conducted to determine the efficacy of 4 Chinese herbal ingredients (CHI) as immune stimulators for an active vaccine in chickens using both in vitro and in vivo assays. The CHI used were Astragalus polysaccharide (APS), Isatis root polysaccharide (IRPS), Propolis polysaccharide, and Epimedium flavone at various concentrations. Two hundred 14-d-old male White Roman chickens were randomly divided into 10 groups. Chickens in groups I to 9 were inoculated with the Newcastle disease virus (NDV) strain IV vaccine by intranasal and intraocular administration. Chickens in groups I to 8 were also administered subcutaneously on the dorsal region of the neck with 0.5 mL of the corresponding CHI at 2 doses: 29 and 58 mg/kg of BW for APS and IRPS and 7.25 and 14.5 mg/kg of BW for the others, once daily for 3 successive days. In group 9 (CHI-free control) and group 10 (both vaccine- and CHI-free control), chickens were injected with 0.5 mL of physiological saline. New-castle disease virus-specific serum hemagglutination inhibition antibody (Ab) production in immunized chickens was quantified using established methods. The results indicate that a majority of the CHI used at appropriate concentrations were effective in enhancing in vitro proliferation of chick embryo fibroblasts in response to the NDV infection. In vivo administration of CHI to vaccinated chickens (7.25 to 58 mg/kg of BW, depending on type) increased serum anti-NDV hemagglutination inhibition Ab titer concentrations, compared with the administration the NDV alone. For all CHI, a beneficial effect on the Ab production was observed on d 21 after the initiation of the vaccination. On the basis of the in vivo doses used, Propolis polysaccharide and Epimedium flavone were more potent than APS and IRPS in promoting the humoral immune response in the young birds (P < 0.05). Collectively, these findings suggest that appropriate doses of CHI can be used as novel, effective immune stimulators for chickens.