Separation of enantiomers by capillary electrophoresis using pentosan polysulfate
Separation of enantiomers by capillary electrophoresis using pentosan polysulfate
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DOI：
10.1002/(sici)1522-2683(19990101)20:1
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发表时间：
1999
期刊：
影响因子：
2.9
通讯作者：
Xiande Wang;J. Lee;D. Armstrong
中科院分区：
文献类型：
--
作者：
Xiande Wang;J. Lee;D. Armstrong
戊聚糖多硫酸酯是一种半合成的多糖，在毛细管电泳中用作手性运行缓冲液添加剂。分离了28种外消旋分析物。分离是成功的，只有在低pH值时，分析物显着质子化。这表明离子相互作用是阴离子戊聚糖多硫酸酯和带正电荷的分析物之间的主要缔合相互作用。与其他线性、基于碳水化合物的手性选择剂（即，硫酸软骨素、肝素和硫酸葡聚糖）戊聚糖多硫酸酯具有阴离子多糖的一些共同特征;但它在结构和性质上有一些差异，这是其不寻常的对映选择性的原因。考察了pH值、磷酸盐缓冲液浓度、戊聚糖多硫酸酯浓度以及有机修饰剂的种类和浓度等因素对分离效果的影响。这些分离的优化取决于分析物的性质，可以通过适当选择实验条件来实现。
Pentosan polysulfate, a semisynthetic polysaccharide, was employed as a chiral run buffer additive in capillary electrophoresis. Twenty‐eight racemic analytes were resolved. The separations were successful only at low pH when the analytes were significantly protonated. This suggests that ionic interactions were the dominant associative interactions between the anionic pentosan polysulfate and the positively charged analytes. Compared to other linear, carbohydrate‐based chiral selectors (i.e., chondroitin sulfates, heparin and dextran sulfate) pentosan polysulfate has some characteristics common of anionic polysaccharides; yet it has several differences in its structure and properties which account for its unusual enantioselectivity. The effects of pH, concentration of phosphate buffer, concentration of pentosan polysulfate and the type and concentration of organic modifier on the enantiomeric separations were investigated. The optimization of these separations were dependent on the nature of the analytes and could be achieved by the proper choice of experimental conditions.