The RNA chaperone Hfq is important for the virulence, motility and stress tolerance in the phytopathogen Xanthomonas campestris
The RNA chaperone Hfq is important for the virulence, motility and stress tolerance in the phytopathogen Xanthomonas campestris
复制标题
RNA 伴侣 Hfq 对于植物病原体野油菜黄单胞菌的毒力、运动性和胁迫耐受性非常重要
DOI：
10.1111/1758-2229.12657
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发表时间：
2018-10-01
影响因子：
3.3
通讯作者：
Tang, Ji-Liang
中科院分区：
文献类型：
--
作者：
Lai, Jie-Ling;Tang, Dong-Jie;Tang, Ji-Liang
RNA伴侣蛋白Hfq已知在细菌的生长和发育中发挥广泛作用。最近，已表明它是许多人类和动物细菌病原体毒力所必需的。尽管有这些研究，但对于Hfq在植物病原细菌中所起的作用知之甚少。在这项研究中，我们表明Hfq是十字花科黑腐病菌野油菜黄单胞菌（Xcc）完全毒力所必需的。我们证明，一个Xcc hfq缺失菌株对中国萝卜的毒力显著减弱，并且在包括胞外酶和胞外多糖在内的毒力因子产生方面表现出严重缺陷。此外，缺乏Hfq的Xcc菌株细胞运动性和应激耐受性显著降低。这些发现表明，Hfq是Xcc毒力和适应性重要方面的关键调节因子。综上所述，我们的研究结果提示存在一个调控网络，使Hfq处于Xcc毒力基因表达控制的中心。
The RNA chaperone, Hfq, is known to play extensive roles in bacterial growth and development. More recently, it has been shown to be required for virulence in many human and animal bacterial pathogens. Despite these studies little is known about the role Hfq plays in phytopathogenic bacteria. In this study, we show Hfq is required for full virulence of the crucifer black rot pathogen Xanthomonas campestris pv. campestris (Xcc). We demonstrate that an Xcc hfq deletion strain is highly attenuated for virulence in Chinese radish and shows a severe defect in the production of virulence factors including extracellular enzymes and extracellular polysaccharide. Furthermore, the Xcc strain lacking Hfq had significantly reduced cell motility and stress tolerance. These findings suggest that Hfq is a key regulator of important aspects of virulence and adaptation of Xcc. Taken together, our findings are suggestive of a regulatory network placing Hfq at the centre of virulence gene expression control in Xcc.