Functionalized Vesicles by Microfluidic Device.
Functionalized Vesicles by Microfluidic Device.
复制标题
通过微流体装置功能化囊泡。
DOI：
10.1007/978-1-4939-6911-1_31
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Lee,Abraham
中科院分区：
文献类型：
--
作者：
Vallejo,Derek;Lee,Shih-Hui;Lee,Abraham
近年来，脂质囊泡已成为用于创建生物传感器的流行载体。囊泡可以将反应组分保持在选择性渗透膜内，该选择性渗透膜为膜蛋白生物传感元件提供了理想的环境。脂质双层允许蛋白质保留其天然结构和功能，并且膜流动性可以允许构象变化和与靶分析物的生理相互作用。在这里，我们提出了两种方法，用于生产的巨单层囊泡（GUV）内的微流体装置，可用作生物传感器的基础。使用非挥发性油相从水包油包水（W/O/W）双乳液模板产生囊泡。为了产生GUV，可以通过用乙醇提取或通过改变油和载体溶液之间的界面张力来去除油，从而使油缩回到结构一侧的帽中，留下暴露的脂质双层。本文还介绍了将传感元件和膜蛋白孔集成到囊泡上的方法。
In recent years, lipid vesicles have become popular vehicles for the creation of biosensors. Vesicles can hold reaction components within a selective permeable membrane that provides an ideal environment for membrane protein biosensing elements. The lipid bilayer allows a protein to retain its native structure and function, and the membrane fluidity can allow for conformational changes and physiological interactions with target analytes. Here, we present two methods for the production of giant unilamellar vesicles (GUVs) within a microfluidic device that can be used as the basis for a biosensor. The vesicles are produced from water-in-oil-in-water (W/O/W) double emulsion templates using a nonvolatile oil phase. To create the GUVs, the oil can be removed via extraction with ethanol, or by altering the interfacial tension between the oil and carrier solution causing the oil to retract into a cap on one side of the structure, leaving behind an exposed lipid bilayer. Methods to integrate sensing elements and membrane protein pores onto the vesicles are also introduced in this work.