RNA-catalyzed RNA polymerization: Accurate and general RNA-templated primer extension
RNA-catalyzed RNA polymerization: Accurate and general RNA-templated primer extension
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DOI：
10.1126/science.1060786
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发表时间：
2001-05-18
期刊：
影响因子：
56.9
通讯作者：
Bartel, DP
中科院分区：
文献类型：
--
作者：
Johnston, WK;Unrau, PJ;Bartel, DP
关于生命早期进化的RNA世界假说依赖于一些RNA序列可以催化RNA复制的前提。为了支持这一猜想，我们在这里描述了一种RNA分子，它催化RNA复制所需的聚合类型。核酶利用三磷酸核苷和RNA模板的编码信息，通过连续添加多达14个核苷酸（超过RNA螺旋的一整圈）来延伸RNA引物。就引物和模板RNA的序列和长度而言，其聚合活性是一般的，条件是引物的3'末端与模板配对。它的聚合也相当准确：当克隆和测序延伸了11个核苷酸的引物时，1100个测序核苷酸中有1088个与模板匹配。
The RNA world hypothesis regarding the early evolution of Life relies on the premise that some RNA sequences can catalyze RNA replication. In support of this conjecture, we describe here an RNA molecule that catalyzes the type of polymerization needed for RNA replication. The ribozyme uses nucleoside triphosphates and the coding information of an RNA template to extend an RNA primer by the successive addition of up to 14 nucleotides-more than a complete turn of an RNA helix. Its polymerization activity is general in terms of the sequence and the Length of the primer and template RNAs, provided that the 3' terminus of the primer pairs with the template. Its polymerization is also quite accurate: when primers extended by 11 nucleotides were cloned and sequenced, 1088 of 1100 sequenced nucleotides matched the template.