Cornea surgery with nanojoule femtosecond laser pulses
Cornea surgery with nanojoule femtosecond laser pulses
复制标题
使用纳焦耳飞秒激光脉冲进行角膜手术
DOI：
10.1117/12.590445
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
K. König
中科院分区：
文献类型：
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作者：
K. König
我们报告了一种新的光学方法，用于（i）LASIK手术中的角膜瓣生成以及（ii）基于纳焦耳飞秒激光脉冲的无角膜瓣基质内屈光手术。利用超小型交钥匙MHz激光谐振腔提供了用于多光子烧蚀的近红外200 fs脉冲。使用系统FemtoCutO（JenLab GmbH，Jena，德国）在800 nm激光波长下，在活体新西兰兔中以高精度实现LASIK瓣和角膜内腔。使用低能量的子-2 nJ的激光脉冲，由于光致分裂和自聚焦效应的附带损害被避免。激光烧蚀系统由快速振镜扫描仪、高数值孔径聚焦光学器件以及灵敏的成像系统组成，并且还提供了荧光细胞器和来自胶原的SHG信号的3D多光子成像的可能性。使用角膜的多光子断层扫描来确定准确的组织内光束位置并可视化眼内激光后效应。通过组织学分析研究了基质间激光消融后长达90天的伤口愈合过程。已检测到受损胶原结构的再生和炎症细胞的迁移。
We report on a novel optical method for (i) flap-generation in LASIK procedures as well as (ii) for flap-free intrastromal refractive surgery based on nanojoule femtosecond laser pulses. The near infrared 200 fs pulses for multiphoton ablation have been provided by ultracompact turn-key MHz laser resonators. LASIK flaps and intracorneal cavities have been realized with high precision within living New Zealand rabbits using the system FemtoCutO (JenLab GmbH, Jena, Germany) at 800 nm laser wavelength. Using low-energy sub-2 nJ laser pulses, collateral damage due to photodisruptive and self-focusing effects was avoided. The laser ablation system consists of fast galvoscanners, focusing optics of high numerical aperture as well as a sensitive imaging system and provides also the possibility of 3D multiphoton imaging of fluorescent cellular organelles and SHG signals from collagen. Multiphoton tomography of the cornea was used to determine the exact intratissue beam position and to visualize intraocular post-laser effects. The wound healing process has been investigated up to 90 days after instrastromal laser ablation by histological analysis. Regeneration of damaged collagen structures and the migration of inflammation cells have been detected.