Surface PEGylation and ligand exchange chemistry of FePt nanoparticles for biological applications
Surface PEGylation and ligand exchange chemistry of FePt nanoparticles for biological applications
复制标题
DOI：
10.1021/cm0507819
复制
发表时间：
2005-09-06
影响因子：
8.6
通讯作者：
Rotello, VM
中科院分区：
文献类型：
--
作者：
Hong, R;Fischer, NO;Rotello, VM
用聚乙二醇端基硫醇和多巴胺配体的混合单分子膜对FePT磁性纳米颗粒进行功能化处理。得到的纳米颗粒在水介质中可溶且稳定，包括水、离子溶液和细胞培养介质。表面的硫醇配体很容易与其他含链末端官能团的硫醇交换。通过配体交换化学合成了具有阳离子或阴离子表面的mNPs，以提供能够结合生物分子的配位体外围。通过在纳米颗粒表面引入带电官能团，实现了阳离子MNPs与DNA的表面结合，以及阴离子MNPs与糜蛋白酶的表面结合。这种方法代表了一种合成功能化FePT纳米颗粒的一般策略，该纳米颗粒在水中形成稳定的溶液，并促进这些磁性FePT纳米颗粒在生物应用中的使用。
FePt magnetic nanoparticles (MNPs) were functionalized with a mixed monolayer of poly(ethylene glycol)-terminated thiol and dopamine ligands. The resulting nanoparticles were soluble and stable in aqueous media, including water, ionic solutions, and cell culture medium. The surface thiol ligands are readily exchanged with other thiols bearing chain-end functionalities. MNPs featuring either a cationic or an anionic surface were synthesized by ligand exchange chemistry to afford ligand peripheries capable of binding biomolecules. Surface binding of cationic MNPs to DNA and anionic MNPs to chymotrypsin was enabled by incorporation of a charged functionality on the nanoparticle surface. This approach represents a general strategy to synthesize functionalized FePt nanoparticles that form stable solutions in water and facilitates the use of these magnetic FePt nanoparticles in biological applications.