Induction of macrophage C-C chemokine expression by titanium alloy and bone cement particles
Induction of macrophage C-C chemokine expression by titanium alloy and bone cement particles
复制标题
DOI：
10.1302/0301-620x.81b1.8884
复制
发表时间：
1999-01-01
影响因子：
--
通讯作者：
Smith, RL
中科院分区：
文献类型：
--
作者：
Nakashima, Y;Sun, DH;Smith, RL
在全关节置换术中，颗粒磨损碎屑与假体周围炎症和松动相关。我们测试了钛合金（Ti合金）和PMMA颗粒对单核细胞/巨噬细胞C-C趋化因子、单核细胞趋化蛋白-1（MCP-1）、单核细胞炎症蛋白-1 α（MIP-1 α）以及活化后调节的正常T细胞表达和分泌蛋白（RANTES）表达的影响。通过免疫组化分析假体周围肉芽肿组织中巨噬细胞趋化因子的表达。用RT-PCR、ELISA和单核细胞迁移法检测了体外暴露于钛合金和PMMA颗粒的人单核/巨噬细胞中趋化因子的表达，结果表明，在关节置换术失败的所有组织标本中，均观察到MCP-1和MIP-1 α的表达，钛合金和PMMA颗粒以剂量和时间依赖的方式增加巨噬细胞中MCP-1和MIP-1 α的表达，而RANTES未检测到。暴露于颗粒后，在细胞中也观察到MCP-1和MIP-1 α的mRNA信号水平。从暴露于钛合金和PMMA颗粒的巨噬细胞中收集的培养基刺激单核细胞迁移，MCP-1和MIP-1 α的抗体：抑制培养基样品的趋化活性。巨噬细胞对磨损颗粒的反应释放C-C趋化因子可能导致全关节置换术中骨-植入物界面的慢性炎症。
Particulate wear debris is associated with I periprosthetic inflammation and loosening in total joint arthroplasty, We tested the effects of titanium alloy (Ti-alloy) and PMMA particles on monocyte/macrophage expression of the C-C chemokines, monocyte chemoattractant protein-1 (MCP-1), monocyte inflammatory protein-1 alpha (MIP-1 alpha), and regulated upon activation normal T expressed and secreted protein (RANTES). Periprosthetic granulomatous tissue was analysed for expression of macrophage chemokines by immunohistochemistry. Chemokine expression in human monocytes/macrophages exposed to Ti-alloy and PMMA particles in vitro was determined by RT-PCR, ELISA and monocyte migration.We observed MCP-1 and MIP-1 alpha expression in all tissue samples from failed arthroplasties, Ti-alloy and PMMA particles increased expression of MCP-1 and MIP-1 alpha in macrophages in vitro in a dose- and time-dependent manner whereas RANTES was not detected. mRNA signal levels for MCP-1 and MIP-1 alpha were also observed in cells after exposure to particles. Monocyte migration was stimulated by culture medium collected from macrophages exposed to Ti-alloy and PMMA particles, Antibodies to MCP-1 and MIP-1 alpha: inhibited chemotactic activity of the culture medium samples.Release of C-C chemokines by macrophages in response to wear particles may contribute to chronic inflammation at the bone-implant interface in total joint arthroplasty.